The First School of Clinical Medicine, Southern Medical University, Guangdong, China.
Department of Obstetrics and Gynecology, The Third Affiliated Hospital, Southern Medical University, Guangdong, China.
J Obstet Gynaecol. 2024 Dec;44(1):2361847. doi: 10.1080/01443615.2024.2361847. Epub 2024 Jun 11.
The vaginal flora has been reported to be associated with human papillomavirus (HPV) infection. The purpose of this study was to investigate the characteristics of the cervical microbiota in patients with HPV infection and to analyse the changes in the vaginal flora and enzyme profiles in females with HPV infection.
We conducted a cross-sectional study involving 206 participants who underwent HPV genotyping, sexually transmitted diseases pathogen testing, cytology examination, and microbiome analysis. Additionally, we collected 115 HPV-negative samples and 48 HPV-positive samples for 16S rRNA amplicon sequencing. The vaginal microbial communities of both groups were analysed for diversity and differences to explore their association with HPV infection.
The abundance of was found to be reduced, while was significantly more prevalent in the HPV + group. In terms of alpha diversity indices, the Shannon index ( = .0036) and Simpson index ( = .02) were higher in the HPV + group compared to the HPV - group, indicating greater community diversity in the HPV + group. Among the 10 sexually transmitted diseases pathogens analysed, Uup3 and Uup6 were significantly associated with HPV infection. Statistically significant differences were observed in Nugent scores and bacterial vaginosis between the two groups ( < .05). In functional analysis, 11 proteins and 13 enzymes were found to be significantly altered in the HPV + group.
Our study demonstrates that disruptions in the vaginal flora are associated with HPV infection. Reduced levels of , increased prevalence of , and abnormal enzyme profiles are closely linked to HPV infection.
阴道菌群已被报道与人类乳头瘤病毒（HPV）感染有关。本研究旨在探讨 HPV 感染患者宫颈微生物群的特征，并分析 HPV 感染女性阴道菌群和酶谱的变化。
我们进行了一项横断面研究，纳入了 206 名接受 HPV 基因分型、性传播疾病病原体检测、细胞学检查和微生物组分析的参与者。此外，我们收集了 115 名 HPV 阴性样本和 48 名 HPV 阳性样本进行 16S rRNA 扩增子测序。分析两组的阴道微生物群落多样性和差异，以探讨其与 HPV 感染的关系。
发现 减少，而 在 HPV + 组中明显更为普遍。在 alpha 多样性指数方面，HPV + 组的 Shannon 指数（ = .0036）和 Simpson 指数（ = .02）高于 HPV - 组，表明 HPV + 组的群落多样性更高。在分析的 10 种性传播疾病病原体中，Uup3 和 Uup6 与 HPV 感染显著相关。两组间 Nugent 评分和细菌性阴道病存在统计学差异（ < .05）。功能分析显示，HPV + 组有 11 种蛋白和 13 种酶显著改变。
本研究表明，阴道菌群失调与 HPV 感染有关。减少的 水平，增加的 流行，以及异常的酶谱与 HPV 感染密切相关。
