College of Plant Science, Jilin University, No.5333 Xi'an Road, Changchun 130062, PR China.
J Agric Food Chem. 2024 Jun 26;72(25):14141-14151. doi: 10.1021/acs.jafc.4c02682. Epub 2024 Jun 12.
The cotton aphid, , is a polyphagous pest that stunts host plant growth via direct feeding or transmitting plant virus. Due to the long-term application of insecticides, has developed different levels of resistance to numerous insecticides. We found that five field populations had evolved multiple resistances to neonicotinoids. To explore the resistance mechanism mediated by uridine diphosphate glycosyltransferases (UGTs), two upregulated UGT genes in these five strains, and , were selected for functional analysis of their roles in neonicotinoid detoxification. Transgenic bioassay results indicated that compared with the control lines, the and overexpression lines were more tolerant to thiamethoxam, imidacloprid, and dinotefuran. Knockdown of and significantly increased sensitivity to thiamethoxam, imidacloprid, and dinotefuran. Molecular docking analysis demonstrated that these neonicotinoids could bind to the active pockets of UGT350C3 and UGT344L7. This study provides functional evidence of neonicotinoid detoxification mediated by UGTs and will facilitate further work to identify strategies for preventing the development of neonicotinoid resistance in insects.
棉蚜，是一种多食性害虫，通过直接取食或传播植物病毒来阻碍宿主植物的生长。由于长期使用杀虫剂，棉蚜已经对多种杀虫剂产生了不同程度的抗性。我们发现，五个田间种群已经对新烟碱类杀虫剂产生了多种抗性。为了探索由尿苷二磷酸糖基转移酶（UGTs）介导的抗性机制，我们选择了这五个菌株中两个上调的 UGT 基因 和 进行功能分析，以研究它们在新烟碱类杀虫剂解毒中的作用。转基因生物测定结果表明，与对照系相比，和 过表达系对噻虫嗪、吡虫啉和噻虫胺的耐受性更强。和 的敲低显著增加了对噻虫嗪、吡虫啉和噻虫胺的敏感性。分子对接分析表明，这些新烟碱类杀虫剂可以与 UGT350C3 和 UGT344L7 的活性口袋结合。这项研究为 UGTs 介导的新烟碱类杀虫剂解毒提供了功能证据，并将有助于进一步确定防止昆虫对新烟碱类杀虫剂产生抗性的策略。
