Yajima A, Wakisaka T, Wada Y, Suzuki M
Gynecol Obstet Invest. 1985;19(2):97-102. doi: 10.1159/000299016.
A study of the tissue and intracellular distribution of estradiol receptors was made of the uterine endometrium of the rat, using an immunofluorescence technique. It was found that nearly all cells of the uterine gland have a uniform distribution of receptors, which does not change throughout the sexual cycle from proestrus to estrus. In contrast, a dense pattern of distribution of estradiol receptors was seen in the endometrial stroma during proestrus, whereas a diffuse pattern was seen during estrus. Intracellularly, the transition from the cytoplasm to the nucleus of estradiol reacting to receptors was found to be positively correlated with the incubation temperature and the length of incubation.
采用免疫荧光技术对大鼠子宫内膜的雌二醇受体进行了组织和细胞内分布研究。发现子宫腺的几乎所有细胞受体分布均匀，在从发情前期到发情期的整个性周期中均无变化。相比之下，发情前期子宫内膜基质中可见雌二醇受体的密集分布模式，而发情期则呈现弥散分布模式。在细胞内，与受体发生反应的雌二醇从细胞质向细胞核的转变被发现与孵育温度和孵育时间呈正相关。
