Department of Genetics, Laboratory of Genetics of Microorganisms, Federal University of Parana, Av. Coronel Francisco Heráclito dos Santos, 100, 81531-980 Curitiba, PR, Brazil.
Pontifical Catholic University of Paraná, Imaculada Conceição St., 1155, 80215-901 Curitiba, PR, Brazil.
Gene. 2024 Nov 15;927:148669. doi: 10.1016/j.gene.2024.148669. Epub 2024 Jun 10.
Bacillus species are extensively documented as plant growth-promoting rhizobacteria, contributing significantly to the enhancement of soil fertility, nutrient recycling, and the control of phytopathogens. Utilizing them as biocontrol agents represents an environmentally friendly strategy, particularly within the rhizospheric community. This study presents the comprehensive genome sequences of three B. velezensis strains (LGMB12, LGMB319, and LGMB426) which were previously isolated from root samples of maize (Zea mays L.), along with a type strain FZB42. The research assesses the capability of the three strains for antagonizing fungi, specifically Fusarium graminearum, Fusarium verticillioides, Colletotrichum graminicola, and Stenocarpella sp. In paired cultures involving maize fungi, treatments containing bacteria B. velezensis exhibited statistically significant differences compared to both negative and positive treatments in terms of antagonism. Furthermore, genome mining techniques were employed to explore their inherent antagonistic potential. The assembly revealed that strains LGMB12, LGMB319, LGMB426, and FZB42 exhibit genome sizes of 4,187,541 bp, 4,244,954 bp, 3,976,537 bp, and 3,990,518 respectively. Their respective G + C content stands at 46.42 %, 46.50 %, 46.51 %, and 46.38 %. Moreover, the genomes present multiple gene clusters responsible for the synthesis of secondary metabolites and carbohydrate-active enzymes (CAZymes). These clusters highlight a diverse array of antibacterial and antifungal properties, complemented by numerous plant growth-promoting genes. These results highlight the potential of B. velezensis LGMB12, LGMB319, and LGMB426 strains as biocontrol and plant growth promotion agents, being promising candidates for further studies in agricultural production, including field trials.
芽孢杆菌属被广泛记录为植物促生根际细菌，对提高土壤肥力、养分循环和控制植物病原菌有重要贡献。利用它们作为生物防治剂是一种环保策略，特别是在根际群落中。本研究介绍了先前从玉米（Zea mays L.）根样中分离出的 3 株贝莱斯芽孢杆菌（LGMB12、LGMB319 和 LGMB426）和 1 株模式菌株 FZB42 的全基因组序列。研究评估了这 3 株菌拮抗真菌（特别是禾谷镰刀菌、串珠镰刀菌、禾谷炭疽菌和 Stenocarpella sp.）的能力。在涉及玉米真菌的配对培养中，含有细菌贝莱斯芽孢杆菌的处理与阴性和阳性处理相比，在拮抗作用方面表现出统计学上的显著差异。此外，还采用基因组挖掘技术来探索它们固有的拮抗潜力。组装结果表明，菌株 LGMB12、LGMB319、LGMB426 和 FZB42 的基因组大小分别为 4,187,541 bp、4,244,954 bp、3,976,537 bp 和 3,990,518 bp，它们的 G+C 含量分别为 46.42%、46.50%、46.51%和 46.38%。此外，基因组中存在多个负责合成次生代谢物和碳水化合物活性酶（CAZymes）的基因簇。这些簇突出了多种抗菌和抗真菌特性，同时还存在许多促进植物生长的基因。这些结果突出了贝莱斯芽孢杆菌 LGMB12、LGMB319 和 LGMB426 菌株作为生物防治和植物生长促进剂的潜力，是农业生产中进一步研究的有前途的候选者，包括田间试验。
