Römermann D, Friedrich B
J Bacteriol. 1985 May;162(2):852-4. doi: 10.1128/jb.162.2.852-854.1985.
Curing of the hydrogenase-specifying megaplasmid pHG indigenous to strains of the facultative lithoautotrophic bacterium Alcaligenes eutrophus was correlated with a loss of denitrifying ability (Nitd). The retransfer of plasmid pHG1 reconstituted the Nitd phenotype. Plasmid-free mutants were still capable of converting some nitrate to nitrite, but they did not metabolize nitrite under anaerobic conditions.
兼性化能自养细菌嗜碱产碱杆菌（Alcaligenes eutrophus）菌株所特有的氢化酶指定大质粒pHG的消除与反硝化能力（Nitd）的丧失相关。质粒pHG1的重新转移恢复了反硝化表型。无质粒突变体仍然能够将一些硝酸盐转化为亚硝酸盐，但它们在厌氧条件下不能代谢亚硝酸盐。
