Department of Clinical Pharmacy, Yokohama University of Pharmacy.
Department of Health Pharmacy, Yokohama University of Pharmacy.
Biol Pharm Bull. 2024;47(6):1144-1147. doi: 10.1248/bpb.b24-00037.
Long non-coding RNAs (lncRNAs) are sequences longer than 200 nucleotides, but they do not encode proteins. Nevertheless, they have significant roles in diverse biological functions. It remains unclear how viral infections trigger the expression of lncRNAs. In our previous research, we revealed a distinct type of lncRNAs with a lifespan under 4 h in human HeLa cells. These short-lived lncRNAs might be associated with numerous regulatory roles. Given their potential impact on human physiology, these short-lived lncRNAs could be key indicators to measure polyinosinic:polycytidylic acid (poly I:C) stimulation. In our recent work, we discovered three lncRNAs: IDI2-AS1, OIP5-AS1, and LITATS1. After exposure to poly I:C, imitating viral assault in human A549 cells, IDI2-AS1 levels dropped significantly while OIP5-AS1 and LITATS1 levels rose markedly. Our results indicate that short-lived lncRNAs respond to poly I:C stimulation, exhibiting substantial changes in expression. This indicates that the understanding the role of lncRNAs in the host response to viral infection and the potential for these molecules to serve as novel therapeutic targets.
长非编码 RNA（lncRNA）是指长度大于 200 个核苷酸但不编码蛋白质的序列。然而，它们在多种生物学功能中具有重要作用。目前尚不清楚病毒感染如何触发 lncRNA 的表达。在我们之前的研究中，我们揭示了一种在人类 HeLa 细胞中寿命不到 4 小时的独特类型的 lncRNA。这些短寿命的 lncRNA 可能与许多调节作用有关。鉴于它们对人类生理学的潜在影响，这些短寿命的 lncRNA 可能是衡量聚肌苷酸：聚胞苷酸（poly I:C）刺激的关键指标。在我们最近的工作中，我们发现了三个 lncRNA：IDI2-AS1、OIP5-AS1 和 LITATS1。在 poly I:C 刺激下，模拟人类 A549 细胞中的病毒攻击，IDI2-AS1 的水平显著下降，而 OIP5-AS1 和 LITATS1 的水平显著上升。我们的结果表明，短寿命的 lncRNA 对 poly I:C 刺激有反应，表达发生了显著变化。这表明，lncRNA 在宿主对病毒感染的反应中的作用以及这些分子作为新型治疗靶点的潜力。
