School of Food Science and Technology, Shaanxi University of Science and Technology, Xi'an 710021, Shaanxi, China.
J Agric Food Chem. 2024 Jun 26;72(25):14337-14348. doi: 10.1021/acs.jafc.4c01584. Epub 2024 Jun 12.
Thymol has efficient bactericidal activity against a variety of pathogenic bacteria, but the bactericidal mechanism against () has rarely been reported. In the current study, we investigated the bactericidal mechanism of thymol against . The Results revealed that 150 μg/mL of thymol had 99.9% bactericidal activity on . Intracellular bursts of reactive oxygen species (ROS), Feaccumulation, lipid peroxidation, and DNA breakage were checked by cell staining. The exogenous addition of HO and catalase promoted and alleviated thymol-induced cell death to a certain extent, respectively, and the addition of the ferroptosis inhibitor Liproxstatin-1 also alleviated thymol-induced cell death, confirming that thymol induced Fenton-reaction-dependent ferroptosis in . Proteomic analysis revealed that relevant proteins involved in ROS production, lipid peroxidation accumulation, and DNA repair were significantly upregulated after thymol treatment. Molecular docking revealed two potential binding sites (amino acids 46H and 42F) between thymol and ferritin, and thymol could promote the release of Fe from ferritin proteins through in vitro interactions analyzed. Therefore, we hypothesized that ferritin as a potential target may mediate thymol-induced ferroptosis in . This study provides new ideas for the development of natural inhibitors for controlling in aquatic products.
百里香酚对多种病原菌具有高效的杀菌活性，但对（）的杀菌机制鲜有报道。本研究探讨了百里香酚对（）的杀菌机制。结果表明，150μg/mL 的百里香酚对（）的杀菌率达到 99.9%。通过细胞染色检测到细胞内活性氧（ROS）爆发、Fe 积累、脂质过氧化和 DNA 断裂。外源性添加 HO 和过氧化氢酶在一定程度上促进和缓解了百里香酚诱导的细胞死亡，铁死亡抑制剂 Liproxstatin-1 的添加也缓解了百里香酚诱导的细胞死亡，证实了百里香酚诱导（）发生 Fenton 反应依赖性的铁死亡。蛋白质组学分析显示，百里香酚处理后与 ROS 产生、脂质过氧化积累和 DNA 修复相关的蛋白明显上调。分子对接显示，百里香酚与铁蛋白之间存在两个潜在的结合位点（氨基酸 46H 和 42F），并且百里香酚可以通过体外相互作用分析促进铁蛋白中 Fe 的释放。因此，我们假设铁蛋白作为潜在的靶标可能介导百里香酚诱导的（）铁死亡。本研究为开发用于控制水产品中（）的天然抑制剂提供了新的思路。
