Clement B, Rissel M, Peyrol S, Mazurier Y, Grimaud J A, Guillouzo A
J Histochem Cytochem. 1985 May;33(5):407-14. doi: 10.1177/33.5.3886779.
Experimental conditions have been designed that permit both extracellular and intracellular immunolocalization of various collagen types and fibronectin in rat liver. The procedure involves paraformaldehyde fixation by perfusion of the organ, use of saponin as a membrane permeabilizing agent, and visualization of the matrix components by indirect immunoperoxidase. Intracellular demonstration of collagens was particularly sensitive to the composition of the fixative and the duration of fixation. Hepatocytes contained fibronectin and types I and IV collagen, whereas fat-storing and endothelial cells evidenced type III collagen in addition. All the components were specifically located in the endoplasmic reticulum and/or the Golgi apparatus.
已经设计了实验条件，可对大鼠肝脏中各种类型的胶原蛋白和纤连蛋白进行细胞外和细胞内免疫定位。该程序包括通过对器官进行灌注用多聚甲醛固定，使用皂苷作为膜通透剂，并通过间接免疫过氧化物酶法对基质成分进行可视化。胶原蛋白的细胞内显示对固定剂的组成和固定时间特别敏感。肝细胞含有纤连蛋白以及I型和IV型胶原蛋白，而储脂细胞和内皮细胞还显示有III型胶原蛋白。所有成分都特异性地定位于内质网和/或高尔基体中。
