Hinthong Woranich, Srisook Thassanee, Tanyong Witawat, Kongngoen Thida, Mahikul Wiriya, Santajit Sirijan, Sookrung Nitat, Indrawattana Nitaya
Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok, 10210, Thailand.
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Heliyon. 2024 May 24;10(11):e31896. doi: 10.1016/j.heliyon.2024.e31896. eCollection 2024 Jun 15.
The Gulf of Thailand provides many services to the Thai population, and human activities may influence the diversity of microorganisms in the seawater. Information of the microorganisms' profile which inhabit the coastline can be used to monitor the water quality. This study aimed to investigate the bacterial community in the waters along the coastline provinces, including Rayong, Chonburi, Prachuap Kiri Khan, and Nakhon Sri Thammarat. Seawater samples were collected at each site, and the conductivity, pH, salinity, temperature, and turbidity were measured. The samples were subjected to whole DNA extraction, 16S rRNA amplification, next-generation sequencing, and statistical analysis to identify the bacterial diversity and analyse the effects of water parameters on the bacterial community. The dominant bacterial phyla found were Proteobacteria, Bacteroidota, and Cyanobacteria. Spearman rank correlation analysis revealed a high correlation of , the NS5 marine group, , , , and with the seawater parameters. The predatory bacteria and were reported among these bacterial communities for the first time in the Gulf of Thailand. Interestingly, , a novel candidate for next-generation probiotics to improve human health, was also found in the sample from Nakhon Sri Thammarat Province. Although the rich-ness and diversity of the bacterial communities differed among sampling sites, it is a possible source of many valuable bacteria for future use.
泰国湾为泰国居民提供了许多服务，而人类活动可能会影响海水中微生物的多样性。栖息在海岸线上的微生物概况信息可用于监测水质。本研究旨在调查罗勇府、春武里府、巴蜀府和洛坤府等沿海省份水域中的细菌群落。在每个采样点采集海水样本，并测量其电导率、pH值、盐度、温度和浊度。对样本进行全基因组DNA提取、16S rRNA扩增、二代测序和统计分析，以确定细菌多样性，并分析水质参数对细菌群落的影响。发现的主要细菌门类有变形菌门、拟杆菌门和蓝细菌门。斯皮尔曼等级相关分析显示，NS5海洋菌群、[此处原文缺失部分内容]、[此处原文缺失部分内容]、[此处原文缺失部分内容]和[此处原文缺失部分内容]与海水参数高度相关。在这些细菌群落中首次报道了捕食性细菌[此处原文缺失部分内容]和[此处原文缺失部分内容]。有趣的是，在洛坤府省的样本中还发现了[此处原文缺失部分内容]，这是一种有望改善人类健康的新型下一代益生菌候选菌株。尽管不同采样点的细菌群落丰富度和多样性存在差异，但它可能是未来许多有价值细菌的来源。
