编码羧肽酶G2的假单胞菌基因在酿酒酵母中的表达。
Expression of the Pseudomonas gene coding for carboxypeptidase G2 in Saccharomyces cerevisiae.
作者信息
Clarke L E, Gibson R F, Sherwood R F, Minton N P
出版信息
J Gen Microbiol. 1985 Apr;131(4):897-903. doi: 10.1099/00221287-131-4-897.
PMID:3886839
Abstract
The Pseudomonas gene coding for carboxypeptidase G2 was introduced into Saccharomyces cerevisiae on an Escherichia coli/yeast shuttle vector pROG5. The level of enzyme activity obtained was independent of the orientation of the gene within the pBR322-derived tetracycline resistance gene of the vector, indicating that expression can occur from a Pseudomonas promoter in yeast.
摘要
编码羧肽酶G2的假单胞菌基因通过大肠杆菌/酵母穿梭载体pROG5导入酿酒酵母中。所获得的酶活性水平与该基因在载体的源自pBR322的四环素抗性基因内的方向无关，这表明表达可以从假单胞菌启动子在酵母中发生。
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