Department of Chemical Technologies, Faculty of Technical Sciences, Burgas "Prof. Dr. Asen Zlatarov" University, No. 1 Y. Yakimov Blvd., 8010 Burgas, Bulgaria.
Z Naturforsch C J Biosci. 2024 Jun 14;79(5-6):149-153. doi: 10.1515/znc-2023-0175. Print 2024 May 27.
Bio-electrochemical Systems (BES), particularly Microbial Fuel Cells (MFC), have emerged as promising technologies in environmental biotechnology. This study focused on optimizing the anode bacterial culture immobilization process to enhance BES performance. The investigation combines and modifies two key immobilization methods: covalent bonding with glutaraldehyde and inclusion in a chitosan gel in order to meet the criteria and requirements of the bio-anodes in MFC. The performance of MFCs with immobilized and suspended cultures was compared in parallel experiments. Both types showed similar substrate utilization dynamics with slight advantage of the immobilized bio-anode considering the lower concentration of biomass. The immobilized MFC exhibited higher power generation and metabolic activity, as well. Probably, this is due to improved anodic respiration and higher coulombic efficiency of the reactor. Analysis of organic acids content supported this conclusion showing significant inhibition of the fermentation products production in the MFC reactor with immobilized anode culture.
生物电化学系统（BES），特别是微生物燃料电池（MFC），已成为环境生物技术中极具前景的技术。本研究专注于优化阳极细菌培养固定化过程，以增强 BES 的性能。该研究结合并改进了两种关键的固定化方法：用戊二醛共价键合和包含在壳聚糖凝胶中，以满足 MFC 中生物阳极的标准和要求。在平行实验中比较了固定化和悬浮培养的 MFC 的性能。两种类型的生物膜都表现出相似的基质利用动力学特性，考虑到较低的生物质浓度，固定化生物阳极具有略微优势。固定化 MFC 还表现出更高的发电和代谢活性。这可能是由于阳极呼吸得到改善，以及反应器的库仑效率更高。有机酸含量分析支持了这一结论，表明固定化阳极培养的 MFC 反应器中发酵产物的产生受到了显著抑制。
