Scientific Research Institute of Beijing Tongrentang Co., Ltd, Beijing 100011, China.
Beijing Zhongyan Tongrentang Pharmaceutical R & D Co., Ltd., National Engineering Research Center for R&D of TCM Multi-ingredient Drugs, Beijing 100000, China.
J Asian Nat Prod Res. 2024 Nov;26(11):1348-1357. doi: 10.1080/10286020.2024.2366007. Epub 2024 Jun 13.
is a traditional Chinese medicine with multiple pharmacological activities. Modern pharmacological research has found that extract has an inhibitory effect on α-glucosidase, however, which component can inhibit the activity of α-glucosidase and its degree of inhibition are unknown. To address this issue, this study used affinity ultrafiltration screening combined with UPLC-ESI-Orbitrap-MS technology to screen α-glucosidase inhibitors in . Using affinity ultrafiltration technology, we obtained the active components, and using UPLC-ESI-Orbitrap-MS technology, we quickly analyzed and identified them. As a result, a total of 8 ingredients were selected as α-glucosidase inhibitors.
是一种具有多种药理活性的中药。现代药理学研究发现， 提取物对 α-葡萄糖苷酶具有抑制作用，但具体哪种成分能够抑制 α-葡萄糖苷酶的活性及其抑制程度尚不清楚。针对这一问题，本研究采用亲和超滤筛选结合 UPLC-ESI-Orbitrap-MS 技术对 中的 α-葡萄糖苷酶抑制剂进行筛选。利用亲和超滤技术获得活性成分，采用 UPLC-ESI-Orbitrap-MS 技术对其进行快速分析和鉴定。结果共筛选出 8 种成分作为 α-葡萄糖苷酶抑制剂。
