de Heer E, Daha M R, Bhakdi S, Bazin H, van Es L A
Kidney Int. 1985 Feb;27(2):388-93. doi: 10.1038/ki.1985.21.
We investigated whether the appearance of various complement components in renal deposits of immune complexes correlated with the development of proteinuria in rats with active Heymann nephritis. Sequential kidney biopsy specimens and serum samples were obtained from Lewis rats immunized with Fx1A in complete Freund's adjuvant. Circulating antibodies against purified auto-antigen renal tubular epithelial glycoprotein, as measured by ELISA, were found in the circulation together with a diffuse granular deposition of IgG1, IgG2a, and IgG2b in the glomeruli within 2 weeks after immunization. Biopsy specimens taken 4 weeks after immunization showed diffuse deposition of C4 and C3, which indicated that activation of complement by the classical pathway had occurred. The detection of the C5b-9 complex of complement in glomerular deposits coincided with the development of abnormal proteinuria indicating that the glomerular damage in this autoimmune disease may be caused by complement-mediated lesions in the glomerular capillary walls.
我们研究了免疫复合物肾沉积物中各种补体成分的出现是否与活动性海曼肾炎大鼠蛋白尿的发生相关。从用弗氏完全佐剂中的Fx1A免疫的Lewis大鼠获取连续的肾脏活检标本和血清样本。免疫后2周内，通过ELISA检测发现循环中存在针对纯化的自身抗原肾小管上皮糖蛋白的循环抗体，同时肾小球内有IgG1、IgG2a和IgG2b的弥漫性颗粒沉积。免疫后4周采集的活检标本显示C4和C3弥漫性沉积，这表明经典途径的补体激活已经发生。肾小球沉积物中补体C5b - 9复合物的检测与异常蛋白尿的发生同时出现，表明这种自身免疫性疾病中的肾小球损伤可能是由肾小球毛细血管壁中补体介导的病变引起的。
