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利用成年斑马鱼及其胚胎对一种杀蚊幼虫转基因藻类（莱茵衣藻）进行口服毒性评估。

An oral toxicity assessment of a mosquito larvicidal transgenic algae (Chlamydomonas reinhardtii) using adult Zebrafish and its embryos.

作者信息

Amjad Fareeha, Khan Hamza, Khan Muhammad Islam, Ayub Sidra, Bhatti Rashid, Pervaiz Rabbia, Malik Kausar, Khan Mohsin Ahmad

机构信息

Nutraceuticals and Microbial Biotechnology Lab, National Center of Excellence in Molecular Biology (CEMB), University of the Punjab, Lahore, Pakistan.

Qarshi University, Lahore, Pakistan.

出版信息

PLoS One. 2024 Jun 13;19(6):e0303352. doi: 10.1371/journal.pone.0303352. eCollection 2024.

DOI:10.1371/journal.pone.0303352
PMID:38870118
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11175461/
Abstract

Mosquito-borne diseases pose a global health threat, with pathogens like Malaria, Dengue fever, and others transmitted by mosquitoes. Our study focuses on evaluating the toxicity of genetically engineered mosquito larvicidal algae (Chlamydomonas reinhardtii) to non-target organisms, specifically Zebrafish. We conducted a 90-day experiment, feeding Zebrafish different combinations of larvicidal algae and commercial fish feed. Statistical analysis revealed no significant differences in mortality, allergenicity, or moribundity among groups. Hematology, molecular analysis, and necropsy showed no physiological differences. Our findings indicate that the transgenic algae (TN72.cry11Ba) had no adverse effects on adult Zebrafish or their larvae. This study confirmed the safety of algae on non-target organisms, such as zebrafish.

摘要

蚊媒疾病对全球健康构成威胁，疟疾、登革热等病原体由蚊子传播。我们的研究重点是评估基因工程杀蚊幼虫藻类（莱茵衣藻）对非靶标生物，特别是斑马鱼的毒性。我们进行了一项为期90天的实验，用杀蚊幼虫藻类和商业鱼饲料的不同组合喂养斑马鱼。统计分析表明，各实验组在死亡率、致敏性或濒死状态方面没有显著差异。血液学、分子分析和尸检显示没有生理差异。我们的研究结果表明，转基因藻类（TN72.cry11Ba）对成年斑马鱼及其幼鱼没有不良影响。这项研究证实了藻类对斑马鱼等非靶标生物的安全性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25ef/11175461/151ce6cee272/pone.0303352.g008.jpg
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