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山楂(大果山楂变种)肤色的高密度遗传图谱和数量性状基因座图谱。

High-density genetic map and quantitative trait loci map of skin color in hawthorn ( bge. Var. major N.E.Br.).

作者信息

Wang Dongsheng, Cheng Beibei, Zhang Jijun

机构信息

Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao, China.

College of Horticulture Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, China.

出版信息

Front Genet. 2024 May 30;15:1405604. doi: 10.3389/fgene.2024.1405604. eCollection 2024.

Abstract

Fruit skin color is an important trait of the hawthorn tree, which has an important influence on fruit quality. Bge. var. Major N.E.Br. Is one of the most widely cultivated varieties in China and has a long history of medicinal use. In recent years, it has attracted the attention of the world due to its nutritional and medicinal values. Skin color is the focus of breeders and food processors. At present, skin color-related genes have still not been mapped. In this study, "Shandong Da Mianqiu" (♀, red skin color), "Da Huang Mianzha" (♂, yellow skin color) and 131 F1 hybrids were used to construct genetic map of hawthorn by RAD-seq, and QTL mapping was performed by combining these features with the hue angle and the observed color. In this study, 13,260 SNP was assigned to 17 linkage groups, with an integrated map covering 2,297.75 cM was constructed. A total of 5 QTLs related to hawthorn skin color were detected on LG1, LG3 and LG15. Whether hue angle or pericarp color acts as phenotype for QTL mapping, the candidate genes include , repeat regions and -like. , and play an important role in the color regulation of Hawthorn skin color. These results lay a solid foundation for QTL mapping and molecular marker-assisted breeding of hawthorn.

摘要

果实果皮颜色是山楂树的一个重要性状,对果实品质有重要影响。大山楂变种(Bge. var. Major N.E.Br.)是中国种植最广泛的品种之一,具有悠久的药用历史。近年来,因其营养和药用价值而受到全世界的关注。果皮颜色是育种者和食品加工者关注的焦点。目前,与果皮颜色相关的基因仍未定位。在本研究中,以“山东大绵球”(♀,红色果皮)、“大黄金楂”(♂,黄色果皮)及其131个F1代杂种为试材,采用RAD-seq技术构建山楂遗传图谱,并结合色相角和观测颜色对这些性状进行QTL定位。本研究共将13260个SNP定位到17个连锁群上,构建了覆盖2297.75 cM的整合图谱。在LG1、LG3和LG15上共检测到5个与山楂果皮颜色相关的QTL。无论色相角还是果皮颜色作为QTL定位的表型,候选基因都包括……重复区域和……样。……、……和……在山楂果皮颜色调控中起重要作用。这些结果为山楂的QTL定位和分子标记辅助育种奠定了坚实基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1501/11169616/3751adb5a23a/fgene-15-1405604-g001.jpg

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