Vadas M A, López A F, Williamson D J
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2503-7. doi: 10.1073/pnas.82.8.2503.
The regulation of expression of two human granulocyte functional antigens (GFA-1 and GFA-2) was examined. N-formylmethionylleucylphenylalanine (fMet-Leu-Phe) caused a rapid, dose-dependent enhancement of the expression of these antigens, 2- to 4-fold within 30 min, but not of another surface structure, beta 2-microglobulin. Pretreatment of the cells with cytochalasin B at 5 micrograms/ml further enhanced the effect of fMet-Leu-Phe on the expression of GFA-2, raising its surface expression 11-fold. Lipopolysaccharide also stimulated the expression of GFA-1 and GFA-2. The effect of lipopolysaccharide was less than that of fMet-Leu-Phe and was more marked on GFA-1 than on GFA-2. Pretreatment of neutrophils with fMet-Leu-Phe not only stimulated their cytotoxic activity against antibody-coated target cells but also increased their capacity to be stimulated by monoclonal antibodies to GFA-1 and GFA-2. These findings show that the expression of functional surface structures on human neutrophils is subject to rapid and selective regulation.
对两种人类粒细胞功能抗原(GFA-1和GFA-2)的表达调控进行了研究。N-甲酰甲硫氨酰亮氨酰苯丙氨酸(fMet-Leu-Phe)可快速、剂量依赖性地增强这些抗原的表达,在30分钟内增加2至4倍,但对另一种表面结构β2-微球蛋白则无此作用。用5微克/毫升的细胞松弛素B预处理细胞,可进一步增强fMet-Leu-Phe对GFA-2表达的作用,使其表面表达增加11倍。脂多糖也可刺激GFA-1和GFA-2的表达。脂多糖的作用小于fMet-Leu-Phe,且对GFA-1的作用比对GFA-2更明显。用fMet-Leu-Phe预处理中性粒细胞,不仅可刺激其对抗体包被靶细胞的细胞毒性活性,还可增加其被针对GFA-1和GFA-2的单克隆抗体刺激的能力。这些发现表明,人类中性粒细胞表面功能结构的表达受到快速且选择性的调控。