Institute of Mass Spectrometry, Zhejiang Engineering Research Center of Advanced Mass spectrometry and Clinical Application, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, China.
Zhenhai Institute of Mass Spectrometry, Ningbo, Zhejiang 315211, China.
Analyst. 2024 Aug 5;149(16):4230-4238. doi: 10.1039/d4an00576g.
Persistent luminescent nanoparticles (PLNPs) are excellent luminescent materials, and near-infrared PLNPs are efficiently applied for biosensing and bioimaging due to their advantages of no excitation, excellent light stability and long afterglow. However, due to interference from the complex environment within organisms, single-mode imaging methods often face limitations in selectivity, sensitivity, and accuracy. Therefore, it is desirable to construct a dual-mode imaging probe strategy with higher specificity and sensitivity for bioimaging. Magnetic resonance imaging (MRI) has been widely used in the field of bioimaging due to its advantages of high resolution, non-radiation and non-invasiveness. Here, by combining near-infrared PLNPs and manganese dioxide (MnO) nanosheets, a sensitive and convenient dual-mode "turn on" bioimaging nanoprobe ZGC@MnO has been developed for long afterglow imaging and MRI of endogenous hydrogen peroxide (HO) in the tumor microenvironment (TME). The monitoring of HO has garnered significant attention due to its crucial role in human pathologies. For the dual-mode "turn on" bioimaging nanoprobe, the near-infrared PLNPs of quasi-spherical ZnGaO:Cr (ZGC) nanoparticles were synthesized as luminophores, and MnO nanosheets were utilized as a fluorescence quencher, carrier and HO recognizer. HO in the TME could reduce MnO nanosheets to Mn for MRI, and ZGC nanoparticles were released for long afterglow imaging. Finally, the ZGC@MnO nanoprobe exhibited a rapid response, an excellent signal-to-noise ratio and a limit of detection of 3.67 nM for endogenous HO in the TME. This dual-mode approach enhances the detection sensitivity for endogenous HO, thereby facilitating the research of endogenous HO-associated diseases and clinical diagnostics.
持久发光纳米粒子（PLNPs）是优异的发光材料，由于其无激发、光稳定性好、余辉时间长等优点，近红外 PLNPs 被有效地应用于生物传感和生物成像。然而，由于生物体内复杂环境的干扰，单模式成像方法在选择性、灵敏度和准确性方面往往存在局限性。因此，构建具有更高特异性和灵敏度的双模式成像探针策略对于生物成像非常理想。磁共振成像（MRI）由于具有高分辨率、非辐射和非侵入性等优点，已广泛应用于生物成像领域。在这里，通过将近红外 PLNPs 和二氧化锰（MnO）纳米片结合起来，开发了一种灵敏方便的双模式“开启”生物成像纳米探针 ZGC@MnO，用于肿瘤微环境（TME）中内源性过氧化氢（HO）的长余辉成像和 MRI。由于 HO 在人类病理学中的重要作用，对其的监测引起了人们的极大关注。对于双模式“开启”生物成像纳米探针，合成了准球形 ZnGaO:Cr（ZGC）纳米粒子作为发光体的近红外 PLNPs，MnO 纳米片作为荧光猝灭剂、载体和 HO 识别器。TME 中的 HO 可以将 MnO 纳米片还原为 Mn 用于 MRI，同时释放 ZGC 纳米粒子进行长余辉成像。最后，ZGC@MnO 纳米探针对 TME 中的内源性 HO 表现出快速响应、优异的信噪比和检测限为 3.67 nM。这种双模式方法提高了内源性 HO 的检测灵敏度，从而促进了内源性 HO 相关疾病的研究和临床诊断。
