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采用 ELISA 法检测脑脊液中食欲素 A 在诊断发作性睡病中的价值。

The value of using ELISA to detect orexin-A in cerebrospinal fluid in the diagnosis of narcolepsy.

机构信息

Department of Neurology, The Second Affiliated Hospital of Nanchang University, Nanchang, People's Republic of China.

Sleep Medicine Center, Department of Respiratory and Critical Care Medicine, Peking University People's Hospital, Beijing, People's Republic of China.

出版信息

Medicine (Baltimore). 2024 Jun 14;103(24):e38539. doi: 10.1097/MD.0000000000038539.

DOI:10.1097/MD.0000000000038539
PMID:38875396
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11175925/
Abstract

Orexin in cerebrospinal fluid (CSF) is a neuropeptide synthesized by a cluster of neurons in the lateral hypothalamus. It mainly functions to maintain arousal, regulate feeding, and participate in reward mechanisms. Radioimmunoassay (RIA) and enzyme-linked immunosorbent assay (ELISA) can detect CSF orexin. At present, RIA is widely used but is limited by various conditions, which is not conducive to its widespread development. We aimed to determine whether ELISA can replace RIA in detecting orexin in CSF. We investigated the results of 20 patients with central disorders of hypersomnolence, including 11 with narcolepsy type 1, 2 with narcolepsy type 2, 5 with idiopathic hypersomnia, and 2 with other causes of somnolence. RIA and ELISA were used to detect CSF orexin, and P values <.05 were considered to be significant. In the narcolepsy and non-narcolepsy type 1 groups, there was no correlation between the RIA and ELISA results (P > .05). In the narcolepsy type 1 group, the ELISA and RIA results were significantly different (P < .05), but this was not observed in the non-narcolepsy type 1 group (P > .05). The accuracy of ELISA to detect CSF orexin was lower than that of RIA (P < .05). ELISA cannot replace RIA in the measurement of CSF orexin, and RIA is recommended as the first choice when narcolepsy is suspected.

摘要

脑脊液中的食欲素是由下丘脑外侧的一簇神经元合成的神经肽。它主要作用是维持觉醒、调节进食，并参与奖励机制。放射免疫分析（RIA）和酶联免疫吸附试验（ELISA）可检测脑脊液中的食欲素。目前，RIA 被广泛应用，但受到各种条件的限制，不利于其广泛发展。我们旨在确定 ELISA 是否可以替代 RIA 检测脑脊液中的食欲素。我们研究了 20 例中枢性嗜睡障碍患者的结果，包括 11 例 1 型发作性睡病、2 例 2 型发作性睡病、5 例特发性嗜睡症和 2 例其他原因引起的嗜睡症。使用 RIA 和 ELISA 检测脑脊液中的食欲素，P 值<.05 被认为有统计学意义。在发作性睡病和非 1 型发作性睡病组中，RIA 和 ELISA 结果之间无相关性（P>.05）。在 1 型发作性睡病组中，ELISA 和 RIA 结果差异有统计学意义（P<.05），但在非 1 型发作性睡病组中则无差异（P>.05）。ELISA 检测脑脊液中食欲素的准确性低于 RIA（P<.05）。ELISA 不能替代 RIA 来测量脑脊液中的食欲素，在怀疑发作性睡病时建议选择 RIA 作为首选方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/1101c7b21c34/medi-103-e38539-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/85f57713fb42/medi-103-e38539-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/6d21443ea0d3/medi-103-e38539-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/eec39935bf4f/medi-103-e38539-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/1101c7b21c34/medi-103-e38539-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/85f57713fb42/medi-103-e38539-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/6d21443ea0d3/medi-103-e38539-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/eec39935bf4f/medi-103-e38539-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1c33/11175925/1101c7b21c34/medi-103-e38539-g004.jpg

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