College of Animal Science and Technology, Anhui Agricultural University,Hefei, Anhui 230036, China; Institute for Agri-Food Standard and Testing, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Institute of Agricultural Animal Husbandry & Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201100, China.
Ecotoxicol Environ Saf. 2024 Jul 15;280:116574. doi: 10.1016/j.ecoenv.2024.116574. Epub 2024 Jun 13.
Aflatoxin B1 (AFB1) is commonly found in feed ingredients and foods all over the world, posing a significant threat to food safety and public health in animals and humans. Lactobacillus salivarius (L. salivarius) was recorded to improve the intestinal health and performance of chickens. However, whether L. salivarius can alleviate AFB1-induced hepatotoxicity in geese was unknown. A total of 300 Lande geese were randomly assigned to five groups: control group, AFB1 low-dose group (L), L. salivarius+AFB1 low-dose group (LL), AFB1 high dosage groups (H), L. salivarius+AFB1 high dosage groups (LH), respectively. The results showed that the concentrations of ALT, AST, and GGT significantly increased after exposure to AFB1. Similarly, severe damage of hepatic morphology was observed including the hepatic structure injury and inflammatory cell infiltration. The oxidative stress was evidenced by the elevated concentrations of MDA, and decreased activities of GSH-Px, GSH and SOD. The observation of immunofluorescence, real-time PCR, and western blotting showed that the expression of PINK1 and the value of LC3II/LC3I were increased, but that of p62 significantly decreased after AFB1 exposure. Moreover, the supplementation of L. salivarius effectively improved the geese performance, ameliorated AFB1-induced oxidative stress, inhibited mitochondrial mitophagy and enhanced the liver restoration to normal level. The present study demonstrated that L. salivarius ameliorated AFB1-induced the hepatotoxicity by decreasing the oxidative stress, and regulating the expression of PINK1/Parkin-mediated mitophagy in the mitochondria of the geese liver. Furthermore, this investigation suggested that L. salivarius might serve as a novel and safe additive for preventing AFB1 contamination in poultry feed.
黄曲霉毒素 B1（AFB1）在世界各地的饲料原料和食品中普遍存在，对动物和人类的食品安全和公共健康构成重大威胁。唾液乳杆菌（L. salivarius）已被记录可改善鸡的肠道健康和性能。然而，唾液乳杆菌是否可以减轻鹅的 AFB1 诱导的肝毒性尚不清楚。将 300 只朗德鹅随机分为 5 组：对照组、AFB1 低剂量组（L）、唾液乳杆菌+AFB1 低剂量组（LL）、AFB1 高剂量组（H）、唾液乳杆菌+AFB1 高剂量组（LH）。结果表明，暴露于 AFB1 后，ALT、AST 和 GGT 的浓度显著升高。同样，观察到严重的肝形态损伤，包括肝结构损伤和炎症细胞浸润。MDA 浓度的升高、GSH-Px、GSH 和 SOD 活性的降低表明存在氧化应激。免疫荧光、实时 PCR 和 Western blot 观察表明，AFB1 暴露后 PINK1 的表达和 LC3II/LC3I 的比值增加，但 p62 的表达显著降低。此外，唾液乳杆菌的补充有效地改善了鹅的性能，改善了 AFB1 诱导的氧化应激，抑制了线粒体自噬，并使肝脏恢复到正常水平。本研究表明，唾液乳杆菌通过降低氧化应激，调节 PINK1/Parkin 介导的鹅肝线粒体自噬的表达，改善了 AFB1 诱导的肝毒性。此外，这项研究表明，唾液乳杆菌可能作为一种新型、安全的添加剂，用于防止家禽饲料中 AFB1 的污染。
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