College of Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, P. R. China.
College of Computer Science, Huanggang Normal University, Huanggang, 438000, P. R. China.
Poult Sci. 2022 Mar;101(3):101651. doi: 10.1016/j.psj.2021.101651. Epub 2021 Dec 8.
Aflatoxin B1 (AFB1) is one of the most toxic mycotoxins. It has been reported that dietary exposure to AFB1 is related to the low growth performance, immunosuppression, and high susceptibility to infectious diseases of chickens. The aim of the present study was to evaluate the protective effects of Lactobacillus salivarius on broiler chickens challenged with AFB1. First, AFB1 degradation ability of Lactobacillus salivarius was measured by a high-performance liquid chromatography (HPLC) method. Then, the Arbor Acres broiler chickens were randomly assigned to experimental groups. The effects of Lactobacillus salivarius supplementation on the growth performance, liver function, and meat quality were measured, and immune response was also determined after vaccination with attenuated infectious bursal disease virus (IBDV) vaccine of broilers challenged with AFB1. Besides, resistance to Salmonella Pullorum infection along with AFB1 exposure was determined in broilers. The results showed that Lactobacillus salivarius could effectively degrade AFB1. Lactobacillus salivarius supplementation improved growth performance, liver function, and meat quality of broilers challenged with AFB1. In addition, Lactobacillus salivarius supplementation resulted in enhanced specific antibody and IFN-γ production, and lymphocyte proliferation in broilers challenged with AFB1 after IBDV vaccine immunization. Furthermore, Lactobacillus salivarius supplementation enhanced Salmonella Pullorum infection resistance in broilers challenged with AFB1. Our results revealed a tremendous potential of Lactobacillus salivarius as feed additive to degrading AFB1 and increasing broilers production performance in poultry production.
黄曲霉毒素 B1（AFB1）是最具毒性的霉菌毒素之一。据报道，膳食中摄入 AFB1 与鸡的生长性能降低、免疫抑制和易患传染病有关。本研究旨在评估唾液乳杆菌对 AFB1 攻毒肉鸡的保护作用。首先，采用高效液相色谱（HPLC）法测定唾液乳杆菌对 AFB1 的降解能力。然后，将阿伯丁-艾克尔斯肉鸡随机分配到实验组。测定唾液乳杆菌补充对肉鸡生长性能、肝功能和肉质的影响，并在 AFB1 攻毒后用减毒传染性法氏囊病病毒（IBDV）疫苗接种测定免疫反应。此外，还测定了 AFB1 暴露时对鸡白痢沙门氏菌感染的抗性。结果表明，唾液乳杆菌能有效降解 AFB1。唾液乳杆菌的补充提高了 AFB1 攻毒肉鸡的生长性能、肝功能和肉质。此外，唾液乳杆菌的补充增强了 AFB1 攻毒肉鸡在 IBDV 疫苗免疫后的特异性抗体和 IFN-γ产生以及淋巴细胞增殖。此外，唾液乳杆菌的补充增强了 AFB1 攻毒肉鸡对鸡白痢沙门氏菌感染的抗性。我们的研究结果表明，唾液乳杆菌作为饲料添加剂具有很大的潜力，可以降解 AFB1 并提高家禽生产中的肉鸡生产性能。
