College of Water Resources and Civil Engineering, China Agricultural University, Beijing 100083, PR China.
College of Water Sciences, Beijing Normal University, Beijing 100875, PR China.
J Hazard Mater. 2024 Aug 15;475:134824. doi: 10.1016/j.jhazmat.2024.134824. Epub 2024 Jun 4.
Organophosphorus pesticides (OPPs) are important chemical stressors in aquatic ecosystems, and they attract increasing more attentions recently. However, the impacts of different OPPs on carbon cycling remain unclear, particularly for those functional-yet-uncultivable microbes. This study investigated the change in lake aquatic microbial communities in the presence of dichlorvos, monocrotophos, omethoate and parathion. All OPPs significantly inhibited biomass (p < 0.05) and the expression of carbon cycle-related cbbLG gene (p < 0.01), and altered aquatic microbial community structure, interaction, and assembly. Variance partitioning analysis showed a stronger impact of pesticide type on microbial biomass and community structure, where pesticide concentration played more significant roles in carbon cycling. From analysis of cbbLG gene and PICRUSt2, Luteolibacter and Verrucomicrobiaceae assimilated inorganic carbon through Wood-Ljungdahl pathway, whereas it was Calvin-Benson-Bassham cycle for Cyanobium PCC-6307. This work provides a deeper insight into the behavior and mechanisms of microbial community change in aquatic system in response to OPPs, and explicitly unravels the impacts of OPPs on their carbon-cycling functions.
有机磷农药（OPPs）是水生生态系统中的重要化学应激物，近年来引起了越来越多的关注。然而，不同 OPPs 对碳循环的影响仍不清楚，特别是对于那些具有功能但尚未培养的微生物。本研究调查了二氯松、单氧松、氧乐果和对硫磷存在时湖泊水生微生物群落的变化。所有 OPPs 均显著抑制生物量（p<0.05）和与碳循环相关的 cbbLG 基因的表达（p<0.01），并改变了水生微生物群落结构、相互作用和组装。方差分解分析表明，农药类型对微生物生物量和群落结构的影响更强，而农药浓度在碳循环中发挥了更重要的作用。从 cbbLG 基因和 PICRUSt2 的分析中可以看出，Luteolibacter 和 Verrucomicrobiaceae 通过 Wood-Ljungdahl 途径同化无机碳，而 Cyanobium PCC-6307 则是 Calvin-Benson-Bassham 循环。这项工作深入了解了水生系统中微生物群落对 OPPs 响应的行为和机制，并明确揭示了 OPPs 对其碳循环功能的影响。
