College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Zhejiang Fisheries Test and Aquatic Disease Prevention Center, Zhejiang Fisheries Technical Extension Center, Hangzhou 310023, China.
Int J Biol Macromol. 2024 Jul;273(Pt 2):133135. doi: 10.1016/j.ijbiomac.2024.133135. Epub 2024 Jun 13.
Largemouth bass (Micropterus salmoides) has emerged as a significant economic fish species, with a rise in Aeromonas veronii infections in farming. However, research on adjuvants for vaccines against A. veronii in largemouth bass remains scarce. In present study, recombinant largemouth bass IL-1β (LbIL-1β) was expressed to explore its adjuvant effect on the A. veronii inactivated vaccine. Following vaccination with recombinant LbIL-1β (rLbIL-1β) and the inactivated A. veronii, higher serum SOD levels and lysozyme activities were observed in largemouth bass from inactivated A. veronii + rLbIL-1β vaccinated group. Furthermore, it was discovered that rLbIL-1β was able to boost the serum-specific antibody levels induced by the inactivated A. veronii. The qRT-PCR analysis revealed that rLbIL-1β also enhanced the expression of IgM, CD4, and MHC II in largemouth bass triggered by the inactivated A. veronii. After challenged with live A. veronii, the outcomes demonstrated that the relative percentage survival (RPS) for largemouth bass resulting from the inactivated A. veronii in combination with rLbIL-1β was 76.67 %, surpassing the RPS of 60 % in the inactivated A. veronii group. Collectively, these findings indicate that rLbIL-1β enhances the protective effect of the A. veronii inactivated vaccine on largemouth bass, showcasing potential as an adjuvant for further development.
大口黑鲈(Micropterus salmoides)已成为一种重要的经济鱼类,其养殖过程中 Aeromonas veronii 感染率上升。然而,关于大口黑鲈抗 A. veronii 疫苗佐剂的研究仍然很少。本研究表达了重组大口黑鲈白细胞介素 1β(LbIL-1β),以探索其对灭活 A. veronii 疫苗的佐剂效应。在重组 LbIL-1β(rLbIL-1β)和灭活 A. veronii 免疫后,灭活 A. veronii+rLbIL-1β 免疫组大口黑鲈血清 SOD 水平和溶菌酶活性较高。此外,发现 rLbIL-1β能够增强灭活 A. veronii 诱导的血清特异性抗体水平。qRT-PCR 分析显示,rLbIL-1β还增强了灭活 A. veronii 触发的大口黑鲈 IgM、CD4 和 MHC II 的表达。用活的 A. veronii 攻毒后,结果表明,灭活 A. veronii 与 rLbIL-1β联合使用后,大口黑鲈的相对存活率(RPS)为 76.67%,高于灭活 A. veronii 组的 60%。总之,这些发现表明 rLbIL-1β增强了灭活 A. veronii 疫苗对大口黑鲈的保护作用,具有作为佐剂进一步开发的潜力。