Institute of Stomatology & Oral Maxilla Facial Key Laboratory, First Medical Center of Chinese PLA General Hospital, Beijing 100853, China; Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department, PLA General Hospital and PLA Medical College, Beijing 100853, China; PLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, Beijing 100048, China; Research Unit of Trauma Care, Tissue Repair and Regeneration, Chinese Academy of Medical Sciences, 2019RU051, Beijing 100048, China.
Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department, PLA General Hospital and PLA Medical College, Beijing 100853, China.
Int J Biol Macromol. 2024 Jul;273(Pt 2):132762. doi: 10.1016/j.ijbiomac.2024.132762. Epub 2024 Jun 13.
Wound dressing diligently facilitate healing by fostering hemostasis, immunoregulation, the angiogenesis, and collagen deposition. Our methodology entails fabricating chitosan-taurine nanoparticles (CS-Tau) through an ionic gelation method. The morphology of CS-Tau was observed utilizing Transmission electron microscopy (TEM), scanning electron microscopy (SEM) and Dynamic Light Scattering (DLS). The nanoparticles are subsequently incorporated into carboxymethyl chitosan hydrogels for crosslinking by EDC-NHS, yielding hydrogel dressings (CMCS-CS-Tau) designed to extend the duration of taurine release. In vitro investigations confirmed that these innovative compound dressings displayed superior biodegradation, biocompatibility, cytocompatibility, and non-toxicity, in addition to possessing anti-inflammatory properties, and stimulating the proliferation and mobility of human umbilical vein endothelial cells (HUVECs). Experiments conducted on mice models with full-thickness skin removal demonstrated that CMCS-CS-Tau efficaciously aided in wound healing by spurring angiogenesis, and encouraging collagen deposition. CMCS-CS-Tau can also minimize inflammation and promote collagen deposition in chronic diabetic wound. Hence, CMCS-CS-Tau promotes both acute and chronic diabetic wound healing. Furthermore, the sustained release mechanism of CMCS-CS-Tau on taurine reveals promising potential for extending its clinical utility in relation to various biological effects of taurine.
敷料通过促进止血、免疫调节、血管生成和胶原蛋白沉积来促进伤口愈合。我们的方法是通过离子凝胶法制备壳聚糖-牛磺酸纳米粒子（CS-Tau）。利用透射电子显微镜（TEM）、扫描电子显微镜（SEM）和动态光散射（DLS）观察 CS-Tau 的形态。然后将纳米粒子掺入羧甲基壳聚糖水凝胶中，通过 EDC-NHS 交联，得到旨在延长牛磺酸释放时间的水凝胶敷料（CMCS-CS-Tau）。体外研究证实，这些创新的复合敷料具有优异的生物降解性、生物相容性、细胞相容性和无毒性，同时具有抗炎特性，并刺激人脐静脉内皮细胞（HUVECs）的增殖和迁移。在全层皮肤切除的小鼠模型上进行的实验表明，CMCS-CS-Tau 通过促进血管生成和促进胶原蛋白沉积来有效促进伤口愈合。CMCS-CS-Tau 还可以减少慢性糖尿病伤口的炎症和促进胶原蛋白沉积。因此，CMCS-CS-Tau 促进急性和慢性糖尿病伤口愈合。此外，CMCS-CS-Tau 对牛磺酸的持续释放机制揭示了其在与牛磺酸的各种生物学效应相关的临床应用中的潜在应用前景。
