Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of TCM, School of Chinese Materia Medica, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Guangdong Provincial Key Laboratory of Translational Cancer Research of Chinese Medicines, Joint International Research Laboratory of Translational Cancer Research of Chinese Medicines, International Institute for Translational Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China.
Protein Expr Purif. 2024 Oct;222:106533. doi: 10.1016/j.pep.2024.106533. Epub 2024 Jun 13.
Artemisia argyi is a traditional medicinal and edible plant, generating various triterpenoids with pharmacological activities, such as anti-virus, anti-cancer, and anti-oxidant. The 2,3-oxidosqualene cyclase family of A. argyi offers novel insights into the triterpenoid pathway, which might contribute to the medicinal value of its tissue extracts. Nevertheless, the biosynthesis of active triterpenoids in Artemisia argyi is still uncertain. In this study, four putative OSC (2,3-oxidosqualene cyclase) genes (AaOSC1-4) were first isolated and identified from A. argyi. Through the yeast heterologous expression system, three AaOSCs were characterized for the biosynthesis of diverse triterpenoids including cycloartenol, β-amyrin, (3S,13R)-malabarica-14(27),17,21-trien-3β-ol, and dammara-20,24-dien-3β-ol. AaOSC1 was a multifunctional dammara-20,24-dien-3β-ol synthase, which yielded 8 different triterpenoids, including tricyclic, and tetracyclic products. AaOSC2 and AaOSC3 were cycloartenol, and β-amyrin synthases, respectively. As a result, these findings provide a deeper understanding of the biosynthesis pathway of triterpenes in A. argyi.
艾草是一种传统的药用和食用植物，产生各种具有药理活性的三萜类化合物，如抗病毒、抗癌和抗氧化。艾草的 2,3-氧化鲨烯环化酶家族为三萜类化合物途径提供了新的见解，这可能有助于其组织提取物的药用价值。然而，艾草中活性三萜类化合物的生物合成仍不确定。在这项研究中，首先从艾草中分离和鉴定了四个假定的 OSC（2,3-氧化鲨烯环化酶）基因（AaOSC1-4）。通过酵母异源表达系统，对三个 AaOSC 进行了表征，用于合成多种三萜类化合物，包括环阿尔廷醇、β-香树脂醇、(3S,13R)-马兰西亚-14(27),17,21-三烯-3β-醇和达玛-20,24-二烯-3β-醇。AaOSC1 是一种多功能的达玛-20,24-二烯-3β-醇合成酶，可生成 8 种不同的三萜类化合物，包括三环和四环产物。AaOSC2 和 AaOSC3 分别是环阿尔廷醇和β-香树脂醇合成酶。因此，这些发现为深入了解艾草中三萜类化合物的生物合成途径提供了依据。
