Graduate Program in Food Science and Technology, Federal Rural University of Rio de Janeiro, Seropédica, Rio de Janeiro (RJ), Brazil.
Embrapa Food Technology, Avenida das Américas, 29501, Rio de Janeiro (RJ) 23020-470, Brazil.
Food Res Int. 2024 Aug;189:114569. doi: 10.1016/j.foodres.2024.114569. Epub 2024 May 28.
Lentil (Lens culinaris) is a protein-rich legume consumed worldwide and it also has the potential to become an alternative source of protein ingredient for human nutrition. The aim of this study was to determine the best processing parameters for the whole grain protein wet extraction, as well as to analyze the techno-functional properties, and physical characteristics of the protein concentrate and its flour. It was also evaluated the application of the concentrate into a fish-like croquette. The processing route was carried out by alkaline extraction and acid precipitation of the proteins where the pH, stirring time and solute:solvent ratio were evaluated. The final dried protein concentrate presented 85% protein on dry basis and a mass yield of 14%. The results were reproducible when tested on a first scaling up test. For the techno-functional properties, solubility, water and oil retention capacities, emulsification and foaming capacities and stability, and gelling capacity were tested. As for the food application into fish-like croquettes, the lentil protein showed similar scores for sensory acceptance, flavor and texture when compared to a commercial clean-taste concentrate. The results observed in this study were compatible to other alternative pulse-protein ingredients on the market, positioning lentil protein as a promising alternative protein source to produce ingredients for the plant-based market.
兵豆(Lens culinaris)是一种富含蛋白质的豆类,在全世界范围内都有食用,它也有可能成为人类营养的蛋白质原料的替代来源。本研究的目的是确定全谷物蛋白质湿法提取的最佳加工参数,并分析蛋白质浓缩物及其面粉的工艺功能特性和物理特性,还评估了浓缩物在仿鱼丸中的应用。该加工路线通过蛋白质的碱性提取和酸沉淀进行,其中评估了 pH 值、搅拌时间和固液比。最终干燥的蛋白质浓缩物在干基上含有 85%的蛋白质,产率为 14%。当在第一次放大试验中进行测试时,结果具有重现性。对于工艺功能特性,测试了溶解度、水和油保持能力、乳化和泡沫能力和稳定性以及胶凝能力。就仿鱼丸的食品应用而言,与商业的无异味浓缩物相比,菜豆蛋白在感官接受度、风味和质地方面的得分相似。本研究中的结果与市场上其他替代豆类蛋白成分相兼容,使菜豆蛋白成为生产植物性市场配料的有前途的替代蛋白来源。