Laboratory of Marine Biochemistry, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 819-0395, Fukuoka, Japan.
Laboratory of Marine Biochemistry, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 819-0395, Fukuoka, Japan.
Dev Comp Immunol. 2024 Oct;159:105212. doi: 10.1016/j.dci.2024.105212. Epub 2024 Jun 13.
CD83 is a costimulatory molecule of antigen-presenting cells (APCs) that plays an important role in eliciting adaptive responses. It is also a well-known surface protein on mature dendritic cells (DCs). Furthermore, monocytes have been reported to differentiate into macrophages and monocyte-derived dendritic cells, which play an important role in innate immunity. CD83 expression affects the activation and maturation of DCs and stimulates cell-mediated immune responses. This study aims to reveal the CD83 expression during monocyte differentiation in teleosts, and the CD83 homologs evolutionary relationship. This study found two distinct CD83 homologs (GbCD83 and GbCD83-L) in ginbuna crucian carp (Gb) and investigated the evolutionary relationship among GbCD83 homologs and other vertebrates and the gene and protein expression levels of the homologs during 4 days of monocyte culture. The phylogenetic tree showed that the two GbCD83 homologs are classified into two distinct branches. Interestingly, only ostariophysians (Gb, common carp, rohu, fathead minnow and channel catfish), but not neoteleosts, mammals, and others, have two CD83 homologs. Morphological observation and colony-stimulating factor-1 receptor (CSF-1R), CD83, CD80/86, and CCR7 gene expressions illustrated that there is a differentiation of monocytes isolated from peripheral blood leukocytes after 4 days. Specifically, gene expression and immunocytochemistry revealed that GbCD83 is mainly expressed on monocytes at the early stage of cell culture, whereas GbCD83-L is expressed in the latter stage. These findings provided the first evidence of differential expression of CD83 homologs during monocytes differentiation in teleost.
CD83 是抗原呈递细胞 (APC) 的共刺激分子，在引发适应性反应中起着重要作用。它也是成熟树突状细胞 (DC) 上的一种众所周知的表面蛋白。此外，单核细胞已被报道分化为巨噬细胞和单核细胞衍生的树突状细胞，它们在先天免疫中起着重要作用。CD83 的表达影响 DC 的激活和成熟，并刺激细胞介导的免疫反应。本研究旨在揭示硬骨鱼类单核细胞分化过程中 CD83 的表达情况，以及 CD83 同源物的进化关系。本研究在金鲫鱼 (Gb) 中发现了两种不同的 CD83 同源物 (GbCD83 和 GbCD83-L)，并研究了 GbCD83 同源物与其他脊椎动物之间的进化关系，以及同源物在单核细胞培养 4 天过程中的基因和蛋白表达水平。系统发育树显示，这两种 GbCD83 同源物分为两个不同的分支。有趣的是，只有硬骨鱼 (Gb、鲤鱼、罗非鱼、食蚊鱼和斑点叉尾鮰) 而不是新鳍鱼、哺乳动物和其他动物具有两种 CD83 同源物。形态学观察和集落刺激因子 1 受体 (CSF-1R)、CD83、CD80/86 和 CCR7 基因表达表明，从外周血白细胞中分离的单核细胞在 4 天后发生了分化。具体来说，基因表达和免疫细胞化学显示，GbCD83 主要在细胞培养早期表达于单核细胞上，而 GbCD83-L 则在后期表达。这些发现为硬骨鱼类单核细胞分化过程中 CD83 同源物差异表达提供了首例证据。
