European Commission, Joint Research Centre (JRC), Retieseweg 111, 2440, Geel, Belgium.
Vrije Universiteit Amsterdam, Amsterdam Institute for Life and Environment (A-LIFE), De Boelelaan 1085, 1081, HV Amsterdam, the Netherlands.
Anal Chim Acta. 2024 Aug 1;1315:342757. doi: 10.1016/j.aca.2024.342757. Epub 2024 May 21.
Chlorinated paraffins (CPs) are industrial chemicals categorised as persistent organic pollutants because of their toxicity, persistency and tendency to long-range transport, bioaccumulation and biomagnification. Despite having been the subject of environmental attention for decades, analytical methods for CPs still struggle reaching a sufficient degree of accuracy. Among the issues negatively impacting the quantification of CPs, the unavailability of well-characterised standards, both as pure substances and as matrix (certified) reference materials (CRMs), has played a major role. The focus of this study was to provide a matrix CRM as quality control tool to improve the comparability of CPs measurement results.
We present the process of certification of ERM®-CE100, the first fish reference material assigned with certified values for the mass fraction of short-chain and medium-chain chlorinated paraffins (SCCPs and MCCPs, respectively). The certification was performed in accordance with ISO 17034:2016 and ISO Guide 35:2017, with the value assignment step carried out via an intercomparison of laboratories of demonstrated competence in CPs analysis and applying procedures based on different analytical principles. After confirmation of the homogeneity and stability of the CRM, two certified values were assigned for SCCPs, depending on the calibrants used: 31 ± 9 μg kg and 23 ± 7 μg kg. The MCCPs certified value was established as 44 ± 17 μg kg. All assigned values are relative to wet weight in the CRM that was produced as a fish paste to enhance similarity to routine biota samples.
The fish tissue ERM-CE100 is the first matrix CRM commercially available for the analysis of CPs, enabling analytical laboratories to improve the accuracy and the metrological traceability of their measurements. The certified CPs values are based on results obtained by both gas and liquid chromatography coupled with various mass spectrometric techniques, offering thus a broad validity to laboratories employing different analytical methods and equipment.
氯化石蜡（CPs）是一种工业化学品，因其毒性、持久性和长距离迁移、生物积累和生物放大的趋势而被归类为持久性有机污染物。尽管几十年来一直受到环境关注，但 CPs 的分析方法仍难以达到足够的准确度。在影响 CPs 定量的问题中，缺乏良好表征的标准，无论是纯物质还是基质（认证）参考物质（CRMs），都起着重要的作用。本研究的重点是提供基质 CRM 作为质量控制工具，以提高 CPs 测量结果的可比性。
我们介绍了 ERM®-CE100 的认证过程，这是第一个被指定为短链和中链氯化石蜡（SCCPs 和 MCCPs）质量分数具有认证值的鱼类参考材料。认证是按照 ISO 17034:2016 和 ISO 指南 35:2017 进行的，赋值步骤是通过具有 CPs 分析能力的实验室间比较来完成的，并采用基于不同分析原理的程序。在确认 CRM 的均匀性和稳定性后，根据所用校准物，为 SCCPs 分配了两个认证值：31±9μg kg 和 23±7μg kg。MCCPs 的认证值为 44±17μg kg。所有分配的值均相对于 CRM 的湿重，该 CRM 是作为鱼糊制成的，以增强与常规生物群样品的相似性。
鱼类组织 ERM-CE100 是第一种可用于分析 CPs 的基质 CRM，使分析实验室能够提高其测量的准确性和计量可追溯性。认证的 CPs 值基于气相和液相色谱与各种质谱技术相结合获得的结果，从而为采用不同分析方法和设备的实验室提供了广泛的有效性。
