College of Animal Science and Technology, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, People's Republic of China.
Pestic Biochem Physiol. 2024 Jun;202:105890. doi: 10.1016/j.pestbp.2024.105890. Epub 2024 Mar 28.
Cytochrome P450 plays a crucial role in regulating insect growth, development, and resisting a variety of stresses. Insect metamorphosis and response to external stress are altered by deleting CYP450 genes. In this study, we identified and analyzed a novel gene of CYP450 family, AccCYP6A13, from Apis cerana cerana, and explored its role in the response of Apis cerana cerana to adverse external stressors. It was found that the expression of AccCYP6A13 was spatiotemporal specificity. The expression level increased with age and reached its highest value in the adult stage. The primarily expressiong location were legs, brain, and epidermis of honeybees. Stress conditions can affect the expression of AccCYP6A13 depending on treatment times. RNA interference experiments have shown that knocking down AccCYP6A13 reduces antioxidant activity and deactivates detoxification enzymes, resulting in oxidative damage accumulation and a decline in detoxification capability in bees, as well as inhibiting numerous antioxidant genes. Additionally, knockdown of the AccCYP6A13 gene in Apis cerana cerana resulted in increased sensitivity to pesticides and increased mortality when treated with neonicotinoid pesticides such as thiamethoxam. AccCYP6A13 overexpression in a prokaryotic system further confirmed its role in resistance to oxidative stress. To summarize, AccCYP6A13 may play an essential role in the normal development and response to environmental stress in Apis cerana cerana. Furthermore, this study contributed to the theoretical understanding of bee resistance biology.
细胞色素 P450 在调节昆虫的生长、发育和抵抗各种应激方面起着至关重要的作用。通过删除 CYP450 基因，昆虫的变态和对外界应激的反应会发生改变。在本研究中，我们从中华蜜蜂中鉴定和分析了一个细胞色素 P450 家族的新基因 AccCYP6A13，并探讨了其在中华蜜蜂对不利环境应激源的反应中的作用。结果发现，AccCYP6A13 的表达具有时空特异性。其表达水平随年龄增长而增加，并在成虫期达到最高值。主要表达部位是蜜蜂的腿、脑和表皮。应激条件会根据处理时间影响 AccCYP6A13 的表达。RNA 干扰实验表明，敲低 AccCYP6A13 会降低抗氧化活性并使解毒酶失活，导致蜜蜂体内氧化损伤积累和解毒能力下降，同时抑制许多抗氧化基因。此外，敲低中华蜜蜂 AccCYP6A13 基因会使其对杀虫剂更加敏感，在接触噻虫嗪等新烟碱类杀虫剂时死亡率增加。在原核系统中过表达 AccCYP6A13 进一步证实了其在抵抗氧化应激中的作用。综上所述，AccCYP6A13 可能在中华蜜蜂的正常发育和对环境应激的反应中发挥重要作用。此外，本研究有助于深入了解蜜蜂的抗逆生物学。
