College of Plant Protection, State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Plant Protection, State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Northwest A&F University, Yangling, Shaanxi 712100, China.
Pestic Biochem Physiol. 2024 Jun;202:105915. doi: 10.1016/j.pestbp.2024.105915. Epub 2024 Apr 16.
The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling is activated by infections of bacteria, fungi, viruses and parasites and mediated cellular and humoral immune responses. In the pea aphid Acyrthosiphon pisum little is known about the function of JAK/STAT signaling in its immune system. In this study, we first showed that expression of genes in the JAK/STAT signaling, including the receptors Domeless1/2, Janus kinase (JAK) and transcriptional factor Stat92E, is up-regulated upon bacteria Escherichia coli and Staphylococcus aureus and fungus Beauveria bassiana infections. After knockdown of expression of these genes by means of dsRNA injection, the aphids harbored more bacteria and suffered more death after infected with E. coli and S. aureus, but showed no significant change after B. bassiana infection. Our study suggests the JAK/STAT signaling contributes to the defense against bacterial infection in the pea aphid.
Janus 激酶/信号转导子和转录激活子（JAK/STAT）信号被细菌、真菌、病毒和寄生虫的感染激活，并介导细胞和体液免疫反应。在豌豆蚜 Acyrthosiphon pisum 中，关于 JAK/STAT 信号在其免疫系统中的功能知之甚少。在这项研究中，我们首先表明，JAK/STAT 信号中的基因表达，包括受体 Domeless1/2、Janus 激酶（JAK）和转录因子 Stat92E，在细菌大肠杆菌和金黄色葡萄球菌以及真菌球孢白僵菌感染后上调。通过 dsRNA 注射敲低这些基因的表达后，蚜虫在感染大肠杆菌和金黄色葡萄球菌后携带更多的细菌并遭受更多的死亡，但在感染白僵菌后没有明显变化。我们的研究表明，JAK/STAT 信号有助于豌豆蚜抵抗细菌感染。
