Biomedical Research Institute of Murcia "Pascual Parrilla", Murcia, Spain.
Experimental Medicine and Surgery Unit, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Madrid, Spain.
Ultrasound Med Biol. 2024 Sep;50(9):1415-1425. doi: 10.1016/j.ultrasmedbio.2024.05.021. Epub 2024 Jun 14.
We propose an ultrasonic treatment for collagenase-induced tendinopathy in rat's Achilles tendon using pulses with a low number of cycles, high acoustic pressure and very low duty cycle.
Twenty rats were used to perform the experiment. Four experimental groups of calcaneal tendons were studied: control (n = 6), sham (n = 4), collagenase-induced tendinopathy (n = 8) and ultrasound-treated collagenase-induced tendinopathy (n = 8). Surgical intervention was performed to expose the tendons prior to collagenase injection. A 1 MHz ultrasonic tansducer with a focusing lens was used. Ultrasonic treatments were used with an average total treatment time of 2.5 min, 20-cycle pulses, pressure amplitude p = 7 MPa, and 0.02% duty cycle. Histopathology of the samples was performed to evaluate nuclear density, acute inflammation, and signs of neovascularization. Collagen (types I and III), elastic fibers, and glycosaminoglycans were also analyzed.
No tendon involvement was found by the surgical process. Ultrasonic treatment is safe, as it does not affect healthy tendons. When collagenase infiltrated animals were treated with US, a clear predominance of type I collagen fibers and a similar collagen ratio profile to that observed in the control and sham groups was observed, with a higher density of elastic fibers compared to the control and sham groups and a significant increase in the density of glycosaminoglycans.
The ultrasound treatment proposed reduces the effects of the artificial collagenase lesion to reach the basal level after 45 d.
我们提出了一种使用低周期数、高声压和极低占空比脉冲的超声处理方法，用于治疗胶原酶诱导的大鼠跟腱腱病。
本实验使用了 20 只大鼠。研究了 4 组跟腱：对照组（n = 6）、假手术组（n = 4）、胶原酶诱导腱病组（n = 8）和超声处理胶原酶诱导腱病组（n = 8）。在注射胶原酶之前，通过手术干预暴露肌腱。使用 1 MHz 超声换能器和聚焦透镜进行超声处理。超声处理的平均总治疗时间为 2.5 分钟，20 个周期的脉冲，压力幅度 p = 7 MPa，占空比为 0.02%。对样本进行组织病理学检查，以评估核密度、急性炎症和新生血管化的迹象。还分析了胶原蛋白（I 型和 III 型）、弹性纤维和糖胺聚糖。
手术过程中未发现肌腱受累。超声处理是安全的，因为它不会影响健康的肌腱。当用 US 处理胶原酶浸润的动物时，观察到 I 型胶原纤维明显占优势，并且胶原比例与对照组和假手术组相似，与对照组和假手术组相比，弹性纤维密度更高，糖胺聚糖密度显著增加。
所提出的超声处理可减少人工胶原酶损伤的影响，在 45 天后达到基础水平。
