School of Biotechnology, Campus 11, Kalinga Institute of Industrial Technology (KIIT) Deemed to be University, Bhubaneswar, Odisha 751024, India.
Section of Extension and Social Sciences, ICAR - Central Tuber Crops Research Institute, Thiruvananthapuram - 695017, Kerala, India.
Methods. 2024 Sep;229:30-40. doi: 10.1016/j.ymeth.2024.06.002. Epub 2024 Jun 14.
The objective of this study was to optimise the millet formulation using Levilactobacillus brevis and to evaluate its anticarcinogenic potential in vitro. The formula was developed in the course of the fermentation of finger millet (Eleusine coracana) using L. brevis MTTC 4460 and optimised by response surface methodology and validation by artificial neural networking (ANN). The optimised millet formulation could be obtained using 2 % of bacterial inoculum, 2 % of glucose, and a fermentation duration of 3.3 days with a yield of 5.98 mg/mL lactic acid and 3.38 log (CFU/mL) viable L. brevis with overall desirability value of 1. The fermented millet formulation exhibited antiproliferative and antimigratory effects on MDA-MB-231 and HCT116 cancer cell lines. In addition, the outcomes observed in western blot analysis revealed that the formulation elicited apoptotic responses mediated by the Bcl-2 family of proteins in MDA-MB-231 and HCT116 cell lines while demonstrating no discernible impact on HEK293 normal cells.
本研究旨在利用短乳杆菌优化小米配方，并评估其体外的抗癌潜力。该配方是在利用短乳杆菌 MTTC 4460 发酵小米（珍珠粟）的过程中开发的，并通过响应面法进行优化，并通过人工神经网络（ANN）进行验证。优化后的小米配方可采用 2%的细菌接种量、2%的葡萄糖和 3.3 天的发酵时间获得，乳酸产量为 5.98mg/mL，短乳杆菌活菌数为 3.38log(CFU/mL)，整体适宜度值为 1。发酵小米配方对 MDA-MB-231 和 HCT116 癌细胞系表现出抗增殖和抗迁移作用。此外，Western blot 分析结果表明，该配方在 MDA-MB-231 和 HCT116 细胞系中诱导了 Bcl-2 蛋白家族介导的凋亡反应，而对 HEK293 正常细胞没有明显影响。
