手性 MoS@BC 纤维膜选择性促进周围神经再生。
Chiral MoS@BC fibrous membranes selectively promote peripheral nerve regeneration.
机构信息
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Department of Orthopedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, China.
出版信息
J Nanobiotechnology. 2024 Jun 17;22(1):337. doi: 10.1186/s12951-024-02493-6.
BACKGROUND
Molybdenum disulfide (MoS) has excellent physical and chemical properties. Further, chiral MoS (CMS) exhibits excellent chiroptical and enantioselective effects, and the enantioselective properties of CMS have been studied for the treatment of neurodegenerative diseases. Intriguingly, left- and right-handed materials have different effects on promoting the differentiation of neural stem cells into neurons. However, the effect of the enantioselectivity of chiral materials on peripheral nerve regeneration remains unclear.
METHODS
In this study, CMS@bacterial cellulose (BC) scaffolds were fabricated using a hydrothermal approach. The CMS@BC films synthesized with L-2-amino-3-phenyl-1-propanol was defined as L-CMS. The CMS@BC films synthesized with D-2-amino-3-phenyl-1-propanol was defined as D-CMS. The biocompatibility of CMS@BC scaffolds and their effect on Schwann cells (SCs) were validated by cellular experiments. In addition, these scaffolds were implanted in rat sciatic nerve defect sites for three months.
RESULTS
These chiral scaffolds displayed high hydrophilicity, good mechanical properties, and low cytotoxicity. Further, we found that the L-CMS scaffolds were superior to the D-CMS scaffolds in promoting SCs proliferation. After three months, the scaffolds showed good biocompatibility in vivo, and the nerve conducting velocities of the L-CMS and D-CMS scaffolds were 51.2 m/s and 26.8 m/s, respectively. The L-CMS scaffolds showed a better regenerative effect than the D-CMS scaffolds. Similarly, the sciatic nerve function index and effects on the motor and electrophysiological functions were higher for the L-CMS scaffolds than the D-CMS scaffolds. Finally, the axon diameter and myelin sheath thickness of the regenerated nerves were improved in the L-CMS group.
CONCLUSION
We found that the CMS@BC can promote peripheral nerve regeneration, and in general, the L-CMS group exhibited superior repair performance. Overall, the findings of this study reveal that CMS@BC can be used as a chiral nanomaterial nerve scaffold for peripheral nerve repair.
背景
二硫化钼（MoS）具有优异的物理和化学性质。此外，手性 MoS（CMS）表现出优异的手性光学和对映选择性效应，CMS 的对映选择性已被研究用于治疗神经退行性疾病。有趣的是，左右手材料对促进神经干细胞向神经元分化有不同的影响。然而，手性材料的对映选择性对周围神经再生的影响尚不清楚。
方法
在这项研究中，采用水热法制备了 CMS@细菌纤维素（BC）支架。用 L-2-氨基-3-苯基-1-丙醇合成的 CMS@BC 薄膜被定义为 L-CMS。用 D-2-氨基-3-苯基-1-丙醇合成的 CMS@BC 薄膜被定义为 D-CMS。通过细胞实验验证了 CMS@BC 支架的生物相容性及其对施万细胞（SCs）的影响。此外，将这些支架植入大鼠坐骨神经缺损部位 3 个月。
结果
这些手性支架表现出高亲水性、良好的机械性能和低细胞毒性。进一步，我们发现 L-CMS 支架在促进 SCs 增殖方面优于 D-CMS 支架。3 个月后，支架在体内表现出良好的生物相容性，L-CMS 和 D-CMS 支架的神经传导速度分别为 51.2 m/s 和 26.8 m/s。L-CMS 支架的再生效果优于 D-CMS 支架。同样，L-CMS 支架的坐骨神经功能指数以及对运动和电生理功能的影响均优于 D-CMS 支架。最后，L-CMS 组再生神经的轴突直径和髓鞘厚度得到改善。
结论
我们发现 CMS@BC 可以促进周围神经再生，总体而言，L-CMS 组表现出更好的修复性能。综上所述，本研究结果表明 CMS@BC 可用作周围神经修复的手性纳米材料神经支架。
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