Ashland Specialties France, 655 Route du Pin Montard, 06904, Sophia Antipolis, France.
In Vitro Cell Dev Biol Anim. 2024 Sep;60(8):832-841. doi: 10.1007/s11626-024-00923-1. Epub 2024 Jun 18.
Since March 2013, animal testing for toxicity evaluation of cosmetic ingredients is banned in Europe. This directive applies to all personal care ingredients including oral ingredients. Gingival in vitro 3D models are commercially available. However, it is essential to develop "in house model" to modulate several parameters to study oral diseases, determine the toxicity of ingredients, test biocompatibility, and evaluate different formulations of cosmetic ingredients. Our expertise in tissue engineering allowed us to reconstruct human oral tissues from normal human gingival cells (fibroblasts and keratinocytes). Indeed, isolation from surgical leftover was performed to culture these gingival cells. These cells keep their endogenous capacity to proliferate allowing reconstruction of equivalent tissue close to in vivo tissue. Reconstruction of gingival epithelium, chorion equivalent, and the combination of these two tissues (full thickness) using primary gingival cells displayed all characteristics of an in vivo gingival model.
自 2013 年 3 月以来，在欧洲，动物毒性评估测试已被禁止用于化妆品成分。此指令适用于所有个人护理成分，包括口服成分。牙龈体外 3D 模型已商业化。然而，开发“内部模型”以调节几个参数来研究口腔疾病、确定成分的毒性、测试生物相容性以及评估化妆品成分的不同配方至关重要。我们在组织工程方面的专业知识使我们能够从正常人类牙龈细胞（成纤维细胞和角质细胞）中重建人类口腔组织。事实上，我们从手术剩余物中分离出来培养这些牙龈细胞。这些细胞保持其内在的增殖能力，允许重建与体内组织接近的等效组织。使用原代牙龈细胞重建牙龈上皮、类绒毛膜等效物以及这两种组织的组合（全厚度）显示了体内牙龈模型的所有特征。
