Nishida K, Ueda S, Sano Y
Histochemistry. 1985;82(2):101-6. doi: 10.1007/BF00708192.
Masked indoleamine cells (MICS) in the area postrema and adjacent areas in the rat were immunohistochemically studied (the peroxidase-antiperoxidase method) using a serotonin antiserum. After pretreatment with nialamide (200-300 mg/kg), immunoreactive MICS could be observed. They were small cells (about 12 micron in diameter) with several processes and were distributed in nearly all parts of the area postrema and also in the nucleus tructus solitarii. Following a single intraventricular injection of 75 micrograms 5,6-dihydroxytryptamine, the immunoreactivity of these cells conspicuously decreased for several days. The submicroscopical structure of the cells was investigated using immunoelectron microscopy. Immunoreactive products were observed in the cytoplasm as particles with a diameter of 25-40 nm and high electron density, but these were not found in the nucleus or cell organelles.
采用抗血清，运用过氧化物酶-抗过氧化物酶法，对大鼠最后区及相邻区域的隐匿性吲哚胺细胞（MICS）进行免疫组织化学研究。用尼亚酰胺（200 - 300毫克/千克）预处理后，可观察到免疫反应性MICS。它们是小细胞（直径约12微米），有多个突起，分布于最后区几乎所有部位以及孤束核。单次脑室内注射75微克5,6 - 二羟基色胺后，这些细胞的免疫反应性在数天内显著降低。利用免疫电子显微镜研究细胞的亚微观结构。在细胞质中观察到免疫反应产物为直径25 - 40纳米、电子密度高的颗粒，但在细胞核或细胞器中未发现。
