Takano-Ohmuro H, Obinata T, Kawashima M, Masaki T, Tanaka T
J Cell Biol. 1985 Jun;100(6):2025-30. doi: 10.1083/jcb.100.6.2025.
It has been demonstrated that embryonic chicken gizzard smooth muscle contains a unique embryonic myosin light chain of 23,000 mol wt, called L23 (Katoh, N., and S. Kubo, 1978, Biochem. Biophys. Acta, 535:401-411; Takano-Ohmuro, H., T. Obinata, T. Mikawa, and T. Masaki, 1983, J. Biochem. (Tokyo), 93:903-908). When we examined myosins in developing chicken ventricular and pectoralis muscles by two-dimensional gel electrophoresis, the myosin light chain (Le) that completely comigrates with L23 was detected in both striated muscles at early developmental stages. Two monoclonal antibodies, MT-53f and MT-185d, were applied to characterize the embryonic light chain Le of striated muscles. Both monoclonal antibodies were raised to fast skeletal muscle myosin light chains; the former antibody is specific to fast muscle myosin light chains 1 and 3, whereas the latter recognizes not only fast muscle myosin light chains but also the embryonic smooth muscle light chain L23. The immunoblots combined with both one- and two-dimensional gel electrophoresis showed that Le reacts with MT-185d but not with MT-53f. These results strongly indicate that Le is identical to L23 and that embryonic chicken skeletal, cardiac, and smooth muscles express a common embryo-specific myosin light chain.
已经证明，鸡胚砂囊平滑肌含有一种独特的分子量为23,000的胚胎肌球蛋白轻链，称为L23（加藤，N.，和S.久保，1978，生物化学与生物物理学报，535:401 - 411；高野-大室，H.，T.小畑，T.三川，和T.正木，1983，生物化学杂志（东京），93:903 - 908）。当我们通过二维凝胶电泳检测发育中的鸡心室肌和胸肌中的肌球蛋白时，在早期发育阶段的两种横纹肌中都检测到了与L23完全共迁移的肌球蛋白轻链（Le）。应用两种单克隆抗体MT - 53f和MT - 185d来表征横纹肌的胚胎轻链Le。这两种单克隆抗体都是针对快肌骨骼肌肌球蛋白轻链产生的；前一种抗体对快肌肌球蛋白轻链1和3具有特异性，而后一种抗体不仅识别快肌肌球蛋白轻链，还识别胚胎平滑肌轻链L23。结合一维和二维凝胶电泳的免疫印迹表明，Le与MT - 185d反应，但不与MT - 53f反应。这些结果有力地表明，Le与L23相同，并且鸡胚骨骼肌、心肌和平滑肌表达一种共同的胚胎特异性肌球蛋白轻链。
