Lv Wei, Zhu Liyong, Tan Lifa, Gu Lei, Wang Hongcheng, Du Xuye, Zhu Bin, Zeng Tuo, Wang Caiyun
School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2024 May 29;13(11):1497. doi: 10.3390/plants13111497.
, a wild blueberry species native to the mountainous regions of southwestern China, is notable for its exceptionally high anthocyanin content, surpassing that of many cultivated varieties and offering significant research potential. Glutathione S-transferases (GSTs) are versatile enzymes crucial for anthocyanin transport in plants. Yet, the GST gene family had not been previously identified in . This study utilized a genome-wide approach to identify and characterize the GST gene family in , revealing 88 GST genes grouped into seven distinct subfamilies. This number is significantly higher than that found in closely related species, with these genes distributed across 12 chromosomes and exhibiting gene clustering. A total of 46 members are classified as tandem duplicates. The gene structure of is relatively conserved among related species, showing closer phylogenetic relations to and evidence of purifying selection. Transcriptomic analysis and qRT-PCR indicated that and were highly expressed in flowers, in leaves, and showed significant expression in ripe and fully mature fruits, paralleling trends seen with anthocyanin accumulation. Subcellular localization identified primarily in the plasma membrane, suggesting a potential role in anthocyanin accumulation in fruits. This study provides a foundational basis for further molecular-level functional analysis of the transport and accumulation of anthocyanins in , enhancing our understanding of the molecular mechanisms underlying anthocyanin metabolism in this valuable species.
[物种名称]是一种原产于中国西南部山区的野生蓝莓品种，以其极高的花青素含量而闻名，超过了许多栽培品种，具有重要的研究潜力。谷胱甘肽S-转移酶（GSTs）是植物中花青素运输所必需的多功能酶。然而，此前尚未在[物种名称]中鉴定出GST基因家族。本研究采用全基因组方法鉴定和表征[物种名称]中的GST基因家族，共鉴定出88个GST基因，分为七个不同的亚家族。这一数量显著高于其近缘物种，这些基因分布在12条染色体上并呈现基因簇。共有46个成员被归类为串联重复基因。[物种名称]的基因结构在相关物种中相对保守，与[对比物种1]和[对比物种2]的亲缘关系更近，且存在纯化选择的证据。转录组分析和qRT-PCR表明，[基因1]和[基因2]在花中高表达，[基因3]在叶中高表达，[基因4]在成熟和完全成熟的果实中显著表达，这与花青素积累趋势一致。亚细胞定位确定[蛋白名称]主要位于质膜上，表明其在[物种名称]果实花青素积累中可能发挥作用。本研究为进一步从分子水平对[物种名称]中花青素的运输和积累进行功能分析提供了基础，增进了我们对这种珍贵物种花青素代谢分子机制的理解。
