Zeng Tuo, Zhu Liyong, Su Wenwen, Gu Lei, Wang Hongcheng, Du Xuye, Zhu Bin, Wang Caiyun, Wu Di
Guizhou Key Laboratory of Forest Cultivation in Plateau Mountain, School of Life Sciences, Guizhou Normal University, Guiyang 550025, China.
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
Plants (Basel). 2024 Dec 22;13(24):3582. doi: 10.3390/plants13243582.
, a calciphilic species native to the mountainous regions of Southwest China, is renowned for its high vitamin C and bioactive components, making it valuable for culinary and medicinal uses. This species exhibits remarkable tolerance to the high-calcium conditions typical of karst terrains. However, the underlying mechanisms of this calcium resilience remain unclear. The Ca/cation antiporter (CaCA) superfamily plays a vital role in the transport of Ca and other cations and is crucial for plant tolerance to metal stress. However, the roles and evolutionary significance of the CaCA superfamily members in remain poorly understood. This study identified 22 CaCA superfamily genes in , categorized into four subfamilies. The gene structures of these show considerable conservation across related species. Selection pressure analysis revealed that all are subject to purifying selection. The promoter regions of these genes contain numerous hormone-responsive and stress-related elements. qRT-PCR analyses demonstrated that H/cation exchanger (CAX) and were highly responsive to Ca stress, cation/Ca exchanger (CCX) to Mg stress, and to Na stress. Subcellular localization indicated that is localized to the plant cell membrane, and its stable transformation in tobacco confirmed its ability to confer enhanced resistance to heavy Ca stresses, highlighting its crucial role in the high-calcium tolerance mechanisms of . This research establishes a foundation for further molecular-level functional analyses of the adaptation mechanisms of to high-calcium environments.
[物种名称]是一种原产于中国西南部山区的喜钙物种，以其高维生素C和生物活性成分而闻名，在烹饪和药用方面具有重要价值。该物种对喀斯特地貌典型的高钙条件表现出显著的耐受性。然而，这种钙耐受性的潜在机制仍不清楚。钙/阳离子反向转运蛋白（CaCA）超家族在钙和其他阳离子的运输中起着至关重要的作用，对植物耐受金属胁迫至关重要。然而，CaCA超家族成员在[物种名称]中的作用和进化意义仍知之甚少。本研究在[物种名称]中鉴定出22个CaCA超家族基因，分为四个亚家族。这些基因的结构在相关物种中表现出相当大的保守性。选择压力分析表明，所有基因都受到纯化选择。这些基因的启动子区域包含许多激素响应和胁迫相关元件。qRT-PCR分析表明，H/阳离子交换蛋白（CAX）[基因名称1]和[基因名称2]对钙胁迫高度响应，阳离子/Ca交换蛋白（CCX）[基因名称3]对镁胁迫高度响应，[基因名称4]对钠胁迫高度响应。亚细胞定位表明，[蛋白名称]定位于植物细胞膜，其在烟草中的稳定转化证实了它能够赋予对高钙胁迫的增强抗性，突出了其在[物种名称]高钙耐受机制中的关键作用。本研究为进一步从分子水平分析[物种名称]对高钙环境的适应机制奠定了基础。
