Thorpe G H, Williams L A, Kricka L J, Whitehead T P, Evans H, Stanworth D R
J Immunol Methods. 1985 May 10;79(1):57-63. doi: 10.1016/0022-1759(85)90391-6.
A novel halophenol-enhanced luminol-peroxide luminescent detection method for horseradish peroxidase has been tested in an enzyme immunoassay for IgE. The luminescent enzyme immunoassay was reproducible (within-batch CV, 3.9-13.2%) and values obtained on serum samples showed good agreement with those obtained by colorimetric enzyme immunoassay. The major advantages of the luminescent detection method are that it is rapid, 30 s compared with 30 min for the colorimetric assay using o-phenylenediamine, and the luminescent signal is intense and stable for several minutes.
