Contractor S F, Holmes-Ievers E, Morgan A, Oakey M, Staines N A
J Immunol Methods. 1985 May 10;79(1):99-108. doi: 10.1016/0022-1759(85)90396-5.
Monoclonal antibodies raised against highly purified placental alkaline phosphatase (PALP) reacted specifically with PALP and were found not to cross-react with the liver, kidney and intestinal enzymes. One of these antibodies (AP-3) was selected to develop an enzyme-binding assay to measure levels of PALP in the sera of 11 women throughout pregnancy. PALP activity rose sharply after 28 weeks of gestation reaching a peak at delivery followed by an immediate drop within 4 days to values approaching non-pregnant levels. The potential of using this assay as an index of placental function to monitor progress of a pregnancy is discussed.
针对高度纯化的胎盘碱性磷酸酶（PALP）产生的单克隆抗体与PALP发生特异性反应，且未发现与肝脏、肾脏和肠道酶发生交叉反应。选择其中一种抗体（AP-3）开发一种酶结合测定法，以测量11名孕妇整个孕期血清中的PALP水平。PALP活性在妊娠28周后急剧上升，在分娩时达到峰值，随后在4天内立即下降至接近非妊娠水平的值。本文讨论了使用该测定法作为胎盘功能指标来监测妊娠进展的潜力。
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