College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Key Laboratory of Low-Carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs, Daqing 163319, China.
Int J Mol Sci. 2024 May 27;25(11):5818. doi: 10.3390/ijms25115818.
The NAC family of transcription factors includes no apical meristem (NAM), transcription activator 1/2 (ATAF1/2), and cup-shaped cotyledon (CUC2) proteins, which are unique to plants, contributing significantly to their adaptation to environmental challenges. In the present study, we observed that the PvNAC52 protein is predominantly expressed in the cell membrane, cytoplasm, and nucleus. Overexpression of in strengthened plant resilience to salt, alkali, osmotic, and ABA stresses. PvNAC52 significantly ( < 0.05) reduced the degree of oxidative damage to cell membranes, proline content, and plant water loss by increasing the expression of , , , , , , and . Moreover, the expression of genes associated with abiotic stress responses, such as , , , , , , and , was enhanced by overexpression. A yeast one-hybrid assay showed that PvNAC52 specifically binds to the cis-acting elements ABRE (abscisic acid-responsive elements, ACGTG) within the promoter. This further suggests that PvNAC52 is responsible for the transcriptional modulation of abiotic stress response genes by identifying the core sequence, ACGTG. These findings provide a theoretical foundation for the further analysis of the targeted cis-acting elements and genes downstream of PvNAC52 in the common bean.
NAC 转录因子家族包括无顶端分生组织（NAM）、转录激活因子 1/2（ATAF1/2）和杯状子叶（CUC2）蛋白，它们是植物所特有的，对植物适应环境挑战有重要贡献。在本研究中，我们观察到 PvNAC52 蛋白主要表达于细胞膜、细胞质和细胞核。过表达 可增强植物对盐、碱、渗透和 ABA 胁迫的抗性。PvNAC52 通过增加 、 、 、 、 、 的表达，显著（<0.05）降低了细胞膜氧化损伤程度、脯氨酸含量和植物水分损失。此外，通过过表达 ，还增强了与非生物胁迫反应相关基因如 、 、 、 、 、 的表达。酵母单杂交试验表明，PvNAC52 特异性结合启动子内的顺式作用元件 ABRE（脱落酸响应元件，ACGTG）。这进一步表明，PvNAC52 通过识别核心序列 ACGTG，负责非生物胁迫响应基因的转录调控。这些发现为进一步分析普通菜豆中 PvNAC52 的靶向顺式作用元件和下游基因提供了理论基础。
