Key Laboratory of National Forestry and Grassland Administration on Grassland Resources and Ecology in the Yellow River Delta, College of Grassland Science, Qingdao Agricultural University, Qingdao 266109, China.
Institute for Agricultural Biosciences, Oklahoma State University, Ardmore, OK 73401, USA.
Int J Mol Sci. 2024 Aug 15;25(16):8908. doi: 10.3390/ijms25168908.
Leaves are a key forage part for livestock, and the aging of leaves affects forage biomass and quality. Preventing or delaying premature leaf senescence leads to an increase in pasture biomass accumulation and an improvement in alfalfa quality. NAC transcription factors have been reported to affect plant growth and abiotic stress responses. In this study, 48 genes potentially associated with leaf senescence were identified in alfalfa under dark or salt stress conditions. A phylogenetic analysis divided into six subgroups based on similar gene structure and conserved motif. These were unevenly distributed in 26 alfalfa chromosomes. The results of the collinearity analysis show that all of the were involved in gene duplication. Some -acting elements related to hormones and stress were screened in the 2-kb promoter regions of . Nine of the genes were subjected to qRT-PCR to quantify their expression and -mediated transient expression to verify their functions. The results indicate that and might be key genes involved in alfalfa leaf senescence. Our findings extend the understanding of the regulatory function of in leaf senescence.
叶片是家畜的一种重要饲料部分，叶片的衰老会影响饲料的生物量和质量。防止或延迟过早的叶片衰老会导致牧草生物量积累增加和苜蓿质量改善。NAC 转录因子已被报道影响植物的生长和非生物胁迫响应。在这项研究中，在黑暗或盐胁迫条件下，鉴定出了 48 个与苜蓿叶片衰老相关的潜在基因。基于相似的基因结构和保守基序，系统发育分析将其分为六个亚组。这些基因不均匀地分布在 26 条苜蓿染色体上。共线性分析的结果表明，所有基因都参与了基因复制。在 的 2-kb 启动子区域筛选到与激素和胁迫相关的一些顺式作用元件。对其中的 9 个基因进行 qRT-PCR 定量分析和 - 介导的瞬时表达验证其功能。结果表明， 和 可能是参与苜蓿叶片衰老的关键 基因。我们的研究结果扩展了对 调节叶片衰老功能的理解。
