Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province, College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China.
Int J Mol Sci. 2024 May 30;25(11):6011. doi: 10.3390/ijms25116011.
Carpel number (CN) is an important trait affecting the fruit size and shape of melon, which plays a crucial role in determining the overall appearance and market value. A unique non-synonymous single nucleotide polymorphism (SNP) in () is responsible for the variation of CN in ssp. (hereafter ), but it has been unclear in ssp. (hereafter ). In this study, one major locus controlling the polymorphism of 5-CN (multi-CN) and 3-CN (normal-CN) in was identified using bulked segregant analysis (BSA-seq). This locus was then fine-mapped to an interval of 1.8 Mb on chromosome 12 using a segregating population containing 1451 progeny. is still present in the candidate region. A new allele of , which contains five other nucleotide polymorphisms, including a non-synonymous SNP in coding sequence (CDS), except the SNP reported in , was identified in . A cis-trans test confirmed that the candidate gene, , contributes to the variation of CNs in . The qRT-PCR results indicate that there is no significant difference in the expression level of in the apical stem between the multi-CN plants and the normal-CN plants. Overall, this study provides a genetic resource for melon fruit development research and molecular breeding. Additionally, it suggests that has undergone similar genetic selection but evolved into an independent allele.
心皮数目（CN）是影响瓜果实大小和形状的重要特征，对整体外观和市场价值起着至关重要的作用。（）中的一个独特的非同义单核苷酸多态性（SNP）是导致 ssp. （以下简称）CN 变异的原因，但在 ssp. （以下简称）中还不清楚。在这项研究中，使用 bulked segregant analysis（BSA-seq）鉴定了控制 5-CN（多-CN）和 3-CN（正常-CN）多态性的一个主要位点。然后，使用包含 1451 个后代的分离群体，将该位点精细映射到 12 号染色体上的 1.8 Mb 区间。仍然存在于候选区域中。在 中发现了一个新的 等位基因，它包含五个其他核苷酸多态性，包括编码序列（CDS）中的非同义 SNP，除了在 中报道的 SNP 之外。顺式-反式测试证实候选基因 导致 中 CN 的变化。qRT-PCR 结果表明，在多-CN 植株和正常-CN 植株的顶茎中， 的表达水平没有显著差异。总体而言，这项研究为瓜果实发育研究和分子育种提供了遗传资源。此外，它表明 经历了类似的遗传选择，但已演变成一个独立的等位基因。
