Department of Ophthalmology, Osaka Kaisei Hospital, Osaka 532-0003, Osaka, Japan.
Department of Ophthalmology, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, Osaka, Japan.
Int J Mol Sci. 2024 May 30;25(11):6045. doi: 10.3390/ijms25116045.
In this immunohistological study on the peripheral retina of 3-year-old beagle dogs, excised retina specimens were immunostained with antibodies against nestin, Oct4, Nanog, Sox2, CDX2, cytokeratin 18 (CK 18), RPE65, and YAP1, as well as hematoxylin and DAPI, two nuclear stains. Our findings revealed solitary cysts of various sizes in the inner retina. Intriguingly, a mass of small round cells with scant cytoplasms was observed in the cavity of small cysts, while many disorganized cells partially occupied the cavity of the large cysts. The small cysts were strongly positive for nestin, Oct4, Nanog, Sox2, CDX2, CK18, and YAP1. RPE65-positive cells were exclusively observed in the tissue surrounding the cysts. Since RPE65 is a specific marker of retinal pigment epithelial (RPE) cells, the surrounding cells of the peripheral cysts were presumably derived from RPE cells that migrated intraretinally. In the small cysts, intense positive staining for nestin, a marker of retinal stem cells, seemed to indicate that they were derived from retinal stem cells. The morphology and positive staining for markers of blastocyst and RPE cells indicated that the small cysts may have formed structures resembling the blastocyst, possibly caused by the interaction between retinal stem cells and migrated RPE cells.
在这项对 3 岁比格犬外周视网膜的免疫组织学研究中，切除的视网膜标本用针对巢蛋白、Oct4、Nanog、Sox2、CDX2、细胞角蛋白 18（CK18）、RPE65 和 YAP1 的抗体以及苏木精和 DAPI 两种核染色剂进行免疫染色。我们的研究结果显示，在内视网膜中存在各种大小的单个囊肿。有趣的是，在小囊肿的腔中观察到一团大小不一的细胞质稀少的小圆形细胞，而许多排列不规则的细胞部分占据了大囊肿的腔。小囊肿对巢蛋白、Oct4、Nanog、Sox2、CDX2、CK18 和 YAP1 呈强阳性。仅在囊肿周围组织中观察到 RPE65 阳性细胞。由于 RPE65 是视网膜色素上皮（RPE）细胞的特异性标志物，因此外周囊肿周围的细胞可能来源于移行至视网膜内的 RPE 细胞。在小囊肿中，视网膜干细胞标志物巢蛋白的强烈阳性染色似乎表明它们来源于视网膜干细胞。胚泡和 RPE 细胞标志物的形态和阳性染色表明，小囊肿可能形成类似于胚泡的结构，可能是由视网膜干细胞和移行的 RPE 细胞之间的相互作用引起的。
