Department of Animal Health, NEIKER-Basque Institute for Agricultural Research and Development, Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain.
Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, Spain.
Int J Mol Sci. 2024 Jun 3;25(11):6165. doi: 10.3390/ijms25116165.
() is the causative agent of bovine tuberculosis (bTb). Genetic selection aiming to identify less susceptible animals has been proposed as a complementary measure in ongoing programs toward controlling infection. However, individual animal phenotypes for bTb based on interferon-gamma (IFNɣ) and its use in bovine selective breeding programs have not been explored. In the current study, IFNɣ production was measured using a specific IFNɣ ELISA kit in bovine purified protein derivative (bPPD)-stimulated blood samples collected from Holstein cattle. DNA isolated from the peripheral blood samples collected from the animals included in the study was genotyped with the EuroG Medium Density bead Chip, and the genotypes were imputed to whole-genome sequences. A genome-wide association analysis (GWAS) revealed that the IFNɣ in response to bPPD was associated with a specific genetic profile (heritability = 0.23) and allowed the identification of 163 SNPs, 72 quantitative trait loci (QTLs), 197 candidate genes, and 8 microRNAs (miRNAs) associated with this phenotype. No negative correlations between this phenotype and other phenotypes and traits included in the Spanish breeding program were observed. Taken together, our results define a heritable and distinct immunogenetic profile associated with strong production of IFNɣ in response to .
牛分枝杆菌（bTb）的病原体是（）。为了识别敏感性较低的动物，已经提出了遗传选择作为正在进行的控制感染计划的补充措施。然而，基于干扰素-γ（IFNɣ）的个体动物对 bTb 的表型及其在牛选择性育种计划中的应用尚未得到探索。在当前的研究中，使用针对牛提纯蛋白衍生物（bPPD）刺激的血液样本的特异性 IFNɣ ELISA 试剂盒测量 IFNɣ 的产生，这些血液样本来自荷斯坦奶牛。从参与研究的动物的外周血样中分离出的 DNA 用 EuroG 中密度珠芯片进行基因分型，并将基因型推断为全基因组序列。全基因组关联分析（GWAS）表明，对 bPPD 的 IFNɣ 反应与特定的遗传特征（遗传力=0.23）相关，并确定了 163 个 SNPs、72 个数量性状基因座（QTL）、197 个候选基因和 8 个与该表型相关的 microRNAs（miRNAs）。没有观察到该表型与西班牙育种计划中包含的其他表型和性状之间存在负相关。综上所述，我们的结果定义了一个可遗传的、独特的免疫遗传特征，与针对的强烈 IFNɣ 产生有关。
