Noh Byeong Gwan, Seo Hyung Il, Park Young Mok, Song Su-Bin, Kim Suk, Hong Seung Baek, Lee Nam Kyung, Lee Jonghyun, Kim Tae In, Kwon Chae Hwa, Ahn Ji Hyun
Department of Surgery, Biomedical Research Institute, Pusan National University Hospital, Pusan National University School of Medicine, Busan 49241, Republic of Korea.
Department of Radiology, Biomedical Research Institute, Pusan National University Hospital, Pusan National University School of Medicine, Busan 49241, Republic of Korea.
Cancers (Basel). 2024 Jun 1;16(11):2120. doi: 10.3390/cancers16112120.
Mucins play a pivotal role in epithelial carcinogenesis; however, their role remains elusive in ampulla of Vater (AoV) cancer, regardless of histological subtype. Therefore, we investigated the clinical significance of MUC1, MUC2, MUC5AC, and MUC6 expression in AoV cancer. : Using samples from 68 patients with AoV cancer, we performed immunohistochemical staining for MUC1, MUC2, MUC5AC, and MUC6 using a tissue microarray. Subsequently, we analyzed their expression patterns in relation to clinicopathological parameters and patient outcomes. : Of the patients, 98.5% exhibited positive expression for MUC1, while MUC2, MUC5AC, and MUC6 were expressed in 44.1%, 47.1%, and 41.2% of the patients, respectively. Correlation analyses between mucin expression and clinicopathological factors revealed no significant associations, except between MUC5AC expression and N stage. Univariate analysis demonstrated significant associations between MUC5AC expression and overall survival (OS). Multivariate analysis further confirmed that MUC5AC expression was a significant predictor of OS, along with the N stage. However, MUC5AC expression was not meaningfully associated with recurrence-free survival (RFS). The patients positive for MUC5AC expression had a considerably shorter OS than those with negative expression. : Our study provides insights into the clinical impact of mucins on AoV cancer, regardless of the histological subtype. Although MUC1 expression is universal, MUC5AC expression is a significant prognostic indicator that correlates with lymph node metastasis and poor OS. These results emphasize the possible utility of MUC5AC as a biomarker for extensive lymph node dissection and the prognostic evaluation of patients with AoV cancer.
黏蛋白在上皮细胞癌变过程中起关键作用；然而，无论组织学亚型如何，它们在 Vater 壶腹（AoV）癌中的作用仍不清楚。因此，我们研究了 MUC1、MUC2、MUC5AC 和 MUC6 在 AoV 癌中的表达的临床意义。：我们使用 68 例 AoV 癌患者的样本，通过组织芯片对 MUC1、MUC2、MUC5AC 和 MUC6 进行免疫组织化学染色。随后，我们分析了它们的表达模式与临床病理参数和患者预后的关系。：在这些患者中，98.5% 的患者 MUC1 呈阳性表达，而 MUC2、MUC5AC 和 MUC6 的表达分别见于 44.1%、47.1% 和 41.2% 的患者。黏蛋白表达与临床病理因素之间的相关性分析显示，除 MUC5AC 表达与 N 分期之间外，无显著关联。单因素分析表明 MUC5AC 表达与总生存期（OS）之间存在显著关联。多因素分析进一步证实，MUC5AC 表达与 N 分期一样，是 OS 的重要预测指标。然而，MUC5AC 表达与无复发生存期（RFS）无明显关联。MUC5AC 表达阳性的患者的 OS 明显短于表达阴性的患者。：我们的研究揭示了黏蛋白对 AoV 癌的临床影响，无论其组织学亚型如何。尽管 MUC1 表达普遍存在，但 MUC5AC 表达是一个重要的预后指标，与淋巴结转移和不良 OS 相关。这些结果强调了 MUC5AC 作为广泛淋巴结清扫和 AoV 癌患者预后评估生物标志物的潜在用途。
