Swiss Center for Electronics and Microtechnology (CSEM), 7302 Landquart, Switzerland.
Sensors (Basel). 2024 May 21;24(11):3280. doi: 10.3390/s24113280.
With the steady increase in allergy prevalence worldwide, there is a strong need for novel diagnostic tools for precise, fast, and less invasive testing methods. Herein, a miniatured fluorescence-based biosensing system is developed for the rapid and quantitative detection of allergen-specific immunoglobulin-E. An antibody-based fluorescence assay in a microfluidic-patterned slide, combined with a custom-made portable fluorescence reader for image acquisition and user-friendly software for the data analysis, enables obtaining results for multiple allergens in just ~1 h with only 80 μL of blood serum. The multiplexed detection of common birch, timothy grass, cat epithelia, house dust mite, and dog epithelia shows quantitative IgE-mediated allergic responses to specific allergens in control serum samples with known total IgE concentration. The responses are verified with different control tests and measurements with a commercial fluorescence reader. These results open the door to point-of-care allergy screening for early diagnosis and broader access and for large-scale research in allergies.
随着全球过敏患病率的稳步上升，对于精确、快速和微创检测方法的新型诊断工具的需求非常强烈。在此，开发了一种基于微型荧光的生物传感系统，用于快速定量检测过敏原特异性免疫球蛋白-E。在微流控图案化载玻片上进行基于抗体的荧光分析，结合定制的便携式荧光读取器进行图像采集和用户友好的软件进行数据分析，仅需 80μL 血清即可在~1 小时内获得多种过敏原的结果。对常见的桦树、梯牧草、猫上皮、屋尘螨和狗上皮的多重检测显示，在具有已知总 IgE 浓度的对照血清样本中，对特定过敏原的 IgE 介导的过敏反应具有定量性。通过不同的对照测试和与商业荧光读取器的测量来验证这些反应。这些结果为即时过敏筛查打开了大门，有助于早期诊断以及更广泛的应用和大规模的过敏研究。
