Agricultural College of Guizhou University, Guizhou University, Guiyang, China.
Institute of Crop Science, Anhui Academy of Agricultural Science, Hefei, China.
Physiol Plant. 2024 May-Jun;176(3):e14394. doi: 10.1111/ppl.14394.
The genic male sterility (GMS) system is an important strategy for generating heterosis in plants. To better understand the essential role of lipid and sugar metabolism and to identify additional candidates for pollen development and male sterility, transcriptome and metabolome analysis of a GMS line of 1205AB in B. napus was used as a case study.
To characterize the GMS system, the transcriptome and metabolome profiles were generated for 24 samples and 48 samples of 1205AB in B. napus, respectively. Transcriptome analysis yielded a total of 156.52 Gb of clean data and revealed the expression levels of 109,541 genes and 8,501 novel genes. In addition, a total of 1,353 metabolites were detected in the metabolomic analysis, including 784 in positive ion mode and 569 in negative ion mode.
A total of 15,635 differentially expressed genes (DEGs) and 83 differential metabolites (DMs) were identified from different comparison groups, most of which were involved in lipid and sugar metabolism. The combination of transcriptome and metabolome analysis revealed 49 orthologous GMS genes related to lipid metabolism and 46 orthologous GMS genes related to sugar metabolism, as well as 45 novel genes.
The transcriptome and metabolome profiles and their analysis provide useful reference data for the future discovery of additional GMS genes and the development of more robust male sterility breeding systems for use in the production of plant hybrids.
基因雄性不育（GMS）系统是植物杂种优势产生的重要策略。为了更好地理解脂质和糖代谢的基本作用，并鉴定花粉发育和雄性不育的其他候选基因，我们以 1205AB 油菜雄性不育系为研究对象，进行了转录组和代谢组分析。
为了描述 GMS 系统，我们分别对油菜 1205AB 的 24 个样本和 48 个样本进行了转录组和代谢组分析。转录组分析共产生了 156.52Gb 的清洁数据，揭示了 109541 个基因和 8501 个新基因的表达水平。此外，代谢组分析共检测到 1353 种代谢物，其中正离子模式下有 784 种，负离子模式下有 569 种。
从不同比较组中鉴定出了 15635 个差异表达基因（DEGs）和 83 个差异代谢物（DMs），其中大多数与脂质和糖代谢有关。转录组和代谢组联合分析揭示了 49 个与脂质代谢相关的同源 GMS 基因和 46 个与糖代谢相关的同源 GMS 基因，以及 45 个新基因。
转录组和代谢组图谱及其分析为未来发现更多的 GMS 基因以及开发更稳健的雄性不育系杂种生产系统提供了有用的参考数据。
