Murase T, Inoue S
Metabolism. 1985 Jun;34(6):531-4. doi: 10.1016/0026-0495(85)90189-1.
The aim of this study is to examine whether insulin regulates the activity of hepatic triglyceride lipase. The activities of both hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL) were measured in plasma after intravenous administration of heparin. Insulin-deficient diabetic rats had normal H-TGL activity. Insulin-treated normal rats had no different enzyme activities from those of controls. Ventromedial hypothalamic (VMH) lesioned rats that became obese and showed hyperinsulinemia, had also normal enzyme activities. From these experiments, it is concluded that H-TGL is not an insulin-dependent enzyme. As far as LPL is concerned, this enzyme was increased in both insulin-treated rats and rats with VMH lesions. This was consistent with the established fact that insulin has a stimulatory effect on adipose tissue LPL. The LPL activity in postheparin plasma was not decreased in insulin-deficient rats. It is speculated that a possible increase in muscle LPL compensated for the low adipose tissue LPL in diabetic rats.
本研究的目的是检测胰岛素是否调节肝甘油三酯脂肪酶的活性。静脉注射肝素后，测定血浆中肝甘油三酯脂肪酶（H-TGL）和脂蛋白脂肪酶（LPL）的活性。胰岛素缺乏的糖尿病大鼠H-TGL活性正常。胰岛素治疗的正常大鼠酶活性与对照组无差异。下丘脑腹内侧（VMH）损伤的大鼠变得肥胖并出现高胰岛素血症，其酶活性也正常。从这些实验得出结论，H-TGL不是一种胰岛素依赖酶。就LPL而言，该酶在胰岛素治疗的大鼠和VMH损伤的大鼠中均增加。这与胰岛素对脂肪组织LPL有刺激作用这一既定事实相符。胰岛素缺乏大鼠肝素后血浆中的LPL活性未降低。推测糖尿病大鼠肌肉LPL可能增加，补偿了脂肪组织LPL的降低。
