Koroleva O N, Shabarova Z A, Bibilashvili R Sh
Mol Biol (Mosk). 1985 Mar-Apr;19(2):516-23.
Nitrocellulose filter binding technique has been used to study the binding of E. coli RNA polymerase to synthetic DNA duplexes (100-200 base pairs), containing the repeating fragments of promoters. It has been shown, that the duplex, containing the repeats of "ideal". Pribnow box forms heparin resistant complexes with enzyme, the stability of which is comparable with that of lacUV5 promoter complexes (the half life is approximately 200 min). The synthetic polynucleotide with repeating trp-promoter-operator sequence less stable complexes with RNA polymerase, the half life of which being 30 min.
硝酸纤维素滤膜结合技术已被用于研究大肠杆菌RNA聚合酶与合成DNA双链体（100 - 200个碱基对）的结合，这些双链体包含启动子的重复片段。结果表明，含有“理想”普里布诺框重复序列的双链体与酶形成抗肝素复合物，其稳定性与lacUV5启动子复合物相当（半衰期约为200分钟）。具有重复色氨酸启动子 - 操纵序列的合成多核苷酸与RNA聚合酶形成的复合物稳定性较差，其半衰期为30分钟。
