Messiha F S
Neurobehav Toxicol Teratol. 1985 Mar-Apr;7(2):189-92.
Specific activities of cytoplasmic NAD-dependent alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were determined in liver and specific brain regions of a mouse strain known for its preference for alcohol C57BL and compared with the albino mouse strain. The specific activities of liver and striatal ADH and ALDH were significantly greater in C57BL strain than in the albino mouse strain used. In both mouse strains, the cerebral cortex, striatum, midbrain and the cerebellum were capable of oxidizing ethanol and acetaldehyde. Administration of ethanol solution 20% (v/v) to C57BL mice, 2.0 g/kg, IP once daily for 8 consecutive days, induced liver ADH activity with a rebound occurring after 14 days of the ethanol treatment. Conversely, administration of identical ethanol dose regimens of ethanol to the albino mice produced a small but statistically significant decline in liver ADH after 14 days of ethanol administration. Furthermore, administration of ethanol for 8 days resulted in a marked decrease of striatal ADH and ALDH activity in both mouse strains. However, this inhibitory effect of ethanol on striatal ADH and ALDH activity was not evident after continued administration of ethanol for 14 days. The results are discussed with reference to available information regarding the relevance of biochemical factors underlying genetic differences in ethanol preference in mouse strains.
在一种以偏好酒精而闻名的小鼠品系C57BL的肝脏和特定脑区中，测定了细胞质中依赖烟酰胺腺嘌呤二核苷酸（NAD）的乙醇脱氢酶（ADH）和乙醛脱氢酶（ALDH）的比活性，并与白化小鼠品系进行了比较。C57BL品系肝脏和纹状体中ADH和ALDH的比活性显著高于所用的白化小鼠品系。在这两种小鼠品系中，大脑皮层、纹状体、中脑和小脑都能够氧化乙醇和乙醛。给C57BL小鼠腹腔注射20%（v/v）的乙醇溶液，剂量为2.0 g/kg，每天一次，连续8天，可诱导肝脏ADH活性，在乙醇处理14天后出现反弹。相反，给白化小鼠注射相同剂量的乙醇，在乙醇给药14天后，肝脏ADH出现了微小但具有统计学意义的下降。此外，连续8天给予乙醇导致两种小鼠品系纹状体中ADH和ALDH活性显著降低。然而，在持续给予乙醇14天后，乙醇对纹状体ADH和ALDH活性的这种抑制作用并不明显。结合有关小鼠品系中乙醇偏好遗传差异背后生化因素相关性的现有信息，对结果进行了讨论。
